My Cart (0 )
-
Previous
TOPFlash Lentiviral Dual-Reporter System
$2,400.00
-
Next
ADCP Assay CD32/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter)
$159.00 – $1,895.00
ADCC Assay CD16/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter)
$159.00 – $1,895.00
Contact Us For ADCC Assay Solutions or Inquire About Exclusive Discount Opportunities
-
High Sensitivity for ADCC Assays
-
Dual-Reporter System (EGFP and GLuc)
-
Ideal for Therapeutic Antibody Screening
The ADCC Assay CD16/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter) is engineered for high-sensitivity assessment of antibody-dependent cellular cytotoxicity (ADCC). Available in high-affinity (V158) and low-affinity (F158) CD16A variants, this cell line features dual reporters: EGFP for precise, single-cell resolution via fluorescence, and Gaussia luciferase for versatile luminescence detection in ADCC assays. Ideal for screening therapeutic antibodies and FcγRIIIa-related research, these cells provide reliable, real-time insights into ADCC activity.
Have questions about this product? Our PhD-level staff is ready to assist. Email info@lipexogen.com
Available Options:
Specifications
Details
The ADCC Assay CD16/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter) is engineered to evaluate antibody-dependent cellular cytotoxicity (ADCC) with high precision. These Jurkat cells express either the high-affinity (V158) or low-affinity (F158) variant of CD16A, an Fcγ receptor crucial for binding IgG antibodies. Upon engagement, the NFAT pathway is activated, driving the expression of dual reporters: EGFP for fluorescence detection and secreted Gaussia luciferase (GLuc) for luminescence in the culture media. This dual-readout system offers flexible and sensitive monitoring of ADCC activity, making it ideal for screening therapeutic monoclonal antibodies for ADCC activity and for Fcγ receptor-related research.
|
|
|
| Jurkat (human leukemia T lymphocytes, non-adherent) | |
| Human | |
| Each vial contains 5 x 10^6 cells in 1 ml of Cell Freezing Medium | |
| Blasticidin | |
| EGFP (fluorescence) and Gaussia luciferase (luminescence) | |
| RPMI-1640, 2 mM L-glutamine, 10% FBS, 100 U/ml penicillin, 100 µg/ml streptomycin | |
| Screened and confirmed mycoplasma-free | |
| Liquid nitrogen vapor phase | |
| Shipped on dry ice (Europe, USA, Canada, Asia) | |
| CD16A expression validated by flow cytometry; ADCC induction confirmed with Anti-CD20 and target cells; Stability for 20 passages verified | |
| High Affinity (V158) and Low Affinity (F158) |
Contact Us For More Information
Product Data:
Figure 1. ADCC Assay CD16/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter) cells were co-cultured with target Raji cells in the presence of 100 ng/ml of anti-CD20 antibody (BioXCell SKU: SIM0008) or an isotype control antibody for 16 hours followed by flow cytometry analysis or luminescence detection of cell culture medium on the Spectramax iD3.
Contact Us For More Information
Key Features:
- Dual-reporter system:
- EGFP for fluorescence, enabling single-cell resolution ADCC detection via flow cytometry.
- Gaussia luciferase (GLuc) for luminescence, allowing high-throughput ADCC measurement in microplate format.
- Endogenous NFAT expression.
- Stable CD16A (FcγRIIIa; V158 or F158 allotype) expression, validated by flow cytometry.
- Proprietary enhancer-driven dual-reporter for enhanced sensitivity in ADCC assays.
Applications:
- Screening anti-CD16 monoclonal antibodies.
- Assessing ADCC potency in mAbs.
- Research on Fc receptor-related pathways.
- Measuring ADCC activity via flow cytometry (EGFP) or luminometer (GLuc).
Quality Control: Each cell line undergoes rigorous validation, including CD16A expression and NFAT pathway activation, confirmed via flow cytometry and ADCC assays. The stability of expression is verified for at least 20 passages, and the cells are guaranteed mycoplasma-free.
Shipping and Storage: Cells are shipped on dry ice and should be stored in liquid nitrogen vapor phase upon receipt for optimal viability.
How to Use: Co-culture the Jurkat cells with target cells and the antibody of interest. Measure NFAT activation through luminescence using Promega’s luciferase substrate reagent (not supplied).
Contents:
- 1 vial containing >1 x 10^6 ADCC Assay CD16/NFAT Jurkat Cells (High or Low Affinity).
- 1 ml of Blasticidin (10 mg/ml).
Materials Required but Not Supplied:
- Media for Cell Culture:
- Thaw Medium: RPMI-1640 with 10% FBS and 1% Penicillin/Streptomycin.
- Growth Medium: RPMI-1640 with 10% FBS, 10 μg/ml Blasticidin
- Materials for Cellular Assays:
- Antibodies
- Target cell lines
- 96-well tissue culture-treated white clear-bottom assay plate.
- Promega’s Renilla Luciferase Assay System (for detecting Gaussia luciferase activity).
Contact Us For More Information
Background:
ADCC is a crucial immune mechanism where effector cells, such as natural killer (NK) cells, lyse target cells coated with antibodies. In this process, CD16A on the effector cells binds to the Fc region of IgG antibodies attached to target cells, leading to activation of the NFAT signaling pathway. This activation triggers the release of cytotoxic molecules that mediate target cell death. The CD16A receptor exists in two forms—high-affinity (V158) and low-affinity (F158)—which influence the strength of the ADCC response. This cell line allows for detailed analysis of these interactions, making it an essential tool for therapeutic antibody research and development.
These products are covered by a Limited Use License. For more information, please email info@lipexogen.com
Similar Products
ADCP Assay CD32/NFAT Jurkat Cell Line (EGFP/GLuc Dual-Reporter)
Product Manual
Product Manual
MSDS
MSDS
Frequently Asked Questions
Frequently Asked Questions