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RXRE/DR1 Reporter Lentivirus
$595.00
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MyoD Reporter Lentivirus
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ATF4 Reporter Lentivirus
$595.00
Fluorescent ATF4 Reporter Lentivirus: High-quality lentiviral reporter system that provides a sensitive fluorescent (GFP or RFP) or luminescent (firefly Luc) readout for ATF4 transcriptional activity in human/mouse cells. The ATF4 reporter contains tandem repeats of the consensus C/EBP/ATF response element (CARE) to which ATF4 binds in its transcriptional activation of the tRNA synthetase family genes in response to amino acid stress. The ATF4 reporter can be used to the amino acid stress response pathway or transcriptional activities of ATF4 in mammalian cells. The lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for use with difficult-to-transfect cells including primary and/or thawed cells. All versions of this product feature a drug selection marker (puro/BSD) to facilitate easy establishment of stable cell lines.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0048 | |
| Amino acid response pathway (C/EBP/ATF4 pathway) | |
| C/EBP/ATF response element (CARE) | |
| Monitor ATF4 activity and activation of the tRNA synthetase family in mammalian cells in response to amino acid stress response. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Product Data
Figure 1 (thumbnail). HEK293 cells were transfected with ATF4-TAG-Puro for ~24 hours, followed by ~12 hour treatment with thapsigargin (0.5 µg/ml, in 10% FBS DMEM), and GFP reporter activity was visualized by fluorescence microscopy.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).