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BTLA1/NFAT Reporter Lentivirus
$895.00
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4-1BB/NFAT Reporter Lentivirus
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CTLA4/NFAT Reporter Lentivirus
$895.00
The CTLA4/NFAT Immunotherapy Reporter Lentivirus is designed to study the inhibitory effects of CTLA4-mediated immune checkpoint signaling in T cells. The construct constitutively expresses the CTLA4 receptor, which binds to B7 ligands on antigen-presenting cells, leading to suppression of T cell activity. This engagement decreases cytosolic Ca2+ levels, reducing NFAT activation and driving a corresponding decrease in reporter output: secreted Gaussia luciferase (GLuc) and GFP. Researchers can also use this system to study receptor engagement inhibition, such as with anti-CTLA4 antibodies, which restore reporter activity by relieving CTLA4-mediated suppression. These reporters enable real-time monitoring of CTLA4 signaling and therapeutic interventions that modulate immune checkpoint activity. To use this system, transduce your desired cells with the all-in-one high-titer purified lentivirus to establish a stable, pathway-specific cell line for immunotherapy and immune checkpoint studies. This product is supplied as high-titer lentiviral particles (third generation), which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells, including primary or thawed cells.
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Available Options:
Specifications
CTLA4/NFAT Reporter Lentivirus Details
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| TRV-0029 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| One vial | |
| 380 μl/vial | |
| 1-2×10^6 TU/vial, depending on the variant | |
| 3×10^8 VP/ml | |
| -80oC | |
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| Promoter | EF1α |
| Human CTLA4 (C-terminal Flag) | |
| NM_005214 | |
| Puromycin (Puro) or Blasticidin (BSD) | |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| NFAT response elements | |
| GFP or RFP plus secreted Gaussia Luc (GLuc), separated by P2A | |
*Transduction units (TU), based on the number of drug resistant or fluorescent HEK293FT cells after transduction with the virus.
Vector Layout
Advantages
- Versatile Readouts Luminescent and fluorescent dual readout provide unprecedented flexibility.
- Open Doors to Discovery Monitor signal pathway activity in vitro or in vivo with secreted GLuc.
- Innovative Technology Utilizes our novel reporter technology with upstream enhancer to boost signal intensity.
- License-free & Affordable Generate your own cell lines with ease, freeing you from constraints of expensive fixed commercial reporter cell lines. The EF1α promoter-driven receptor/ligand provides long-term stable expression in cell lines.
- Unmatched customization Various reporters and selection markers to choose from, perfectly tailored to your specific research needs.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of potential customization options are shown in the table below and can be custom-made upon request. Please note that not all options may be feasible due to size limitations and this will depend on the specific product. We also welcome requests for custom products like the one shown on this page.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Lentiviral Transduction Protocol
MSDS
Frequently Asked Questions
Frequently Asked Questions
Can I purchase the plasmid for this product?
TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.