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GAL4-DBD-NR1D1-LBD-VP16-BSD
Price range: $595.00 through $795.00
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OCT4 Reporter Lentivirus
$595.00
FOXA1/2 Reporter Lentivirus
$595.00
FOXA1/2 Reporter Lentivirus: A high-quality lentiviral reporter system designed to detect transcriptional activity mediated by Forkhead box protein A1 (FOXA1) and FOXA2 (also known as HNF-3A and HNF-3B) in mammalian cells. This reporter incorporates tandem repeats of consensus DNA-binding elements within the human protein C promoter to monitor FOXA1/2 transcriptional activity with high specificity. FOXA1 and FOXA2 are pioneering transcription factors that bind condensed chromatin to facilitate the recruitment of other transcription factors. They play crucial roles in organogenesis, differentiation, glycolipid metabolism, proliferation, migration, invasion, and drug resistance. The FOXA1/2 reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, ensuring optimal performance for transducing primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0122 | |
| FOXA signaling pathway | |
| FOXA response elements (HNF3 binding sites) | |
| Monitor FOXA1/2 activity in mammalian cells, screen for activators or inhibitors of FOXA1/2 mediated transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Product Data
Figure 1. GFP Reporter activation of FOXA1/2-TAG-Puro. HEK293FT cells were co-transfected with plasmids for FOXA1/2-TAG-Puro along with empty vector (pcDNA), cDNA encoding human FOXA1, or cDNA encoding human FOXA2 for 36 h before acquiring fluorescent microscopy images. The FOXA2 expression vector contains RFP downstream of the gene, separated by P2A. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. Featured cDNA expression vectors are also available upon request. For more information, please email info@lipexogen.com.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Background
FOXA1 and FOXA2 are essential regulators of transcription, acting as pioneering factors that open condensed chromatin to enable transcriptional machinery access. They play central roles in biological processes such as organ development, differentiation, and metabolic regulation. FOXA1/2 can exert complementary or synergistic effects, influencing cell proliferation, migration, invasion, and drug resistance.
In prostate cells, FOXA1 interacts with the androgen receptor (AR) to regulate transcription of prostate-specific genes. Dysregulation of FOXA1/2 activity is implicated in cancer progression and drug resistance, making them valuable targets for therapeutic research. The FOXA1/2 reporter system provides a versatile tool to study these transcription factors, enabling high-throughput screening of activators and inhibitors and offering insights into their roles in health and disease.
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).