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HOXB13 Reporter Lentivirus
$595.00
FOXN1 Reporter Lentivirus
$595.00
FOXN1 Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse Forkhead box N1 (FOXN1) in mammalian cells. There are nearly 50 Forkhead TFs in the human genome, which share a similar core DNA binding element but differ in flanking sequences that confer binding specificity to different Forkhead TFs. The FOXN1 reporter utilizes transcriptional response elements designed to preferentially readout transcriptional activity of FOXN1 vs other Forkhead TFs. Tandem repeats of the FOXN1 DNA-binding elements allow for the detection of FOXN1 activity. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying FOXN1 activity in difficult-to-transfect cells including primary and/or thawed cells.
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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0062 | |
| Forkhead Transcription Factors (FOXN1) | |
| FOXN1 | |
| Monitor FOXN1 transcriptional activity in mammalian cells, screen for activators or inhibitors of FOXN1 signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. There are nearly 50 forkhead transcription factors (TFs) encoded in the human genome, all of which share a similar core DNA binding element, but differ in the flanking sequences that confer binding specificity for different forkhead TFs. The FOXN1 reporter utilizes forkhead TF response elements that have been optimized preferential binding of FOXN1 vs other forkhead TFs. | |
| Human/mouse |
Product Data
Figure 1 (thumbnail). GFP reporter activation in cells co-transfected with FOXN1-TAG-Puro reporter and FOXN1. HEK293FT cells were transfected with FOXN1-TAG-Puro plasmid along with empty vector (pcDNA) or an expression vector for human FOXN1. After 24-36 h, images were acquired by fluorescence microscopy. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. Human FOXN1 cDNA expression vector is also available upon request. For more information, please email us.
Figure 2. HEK293FT cells were transfected with FOXN1-TAG-Puro along with an empty vector, wild-type FOXN1-HA, or a TAD mutant of FOXN1 (FOXN1-Mt-HA) for 32 hours before fluorescence microscopy. To order FOXN1 or FOXN1-Mt cDNA vectors, please email us.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
- High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb