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GITR/NFκB Reporter Lentivirus
$895.00
The GITR/NF-κB Immunotherapy Reporter Lentivirus is designed to measure the co-stimulation effect of GITR on T-cell activation. Upon binding by an agonist antibody or GITR ligand expressed on target cells, GITR on effector cells activates the NF-κB signaling pathway, enhancing T cell activation and immunity against target cells, such as tumor cells. This reporter lentivirus is an efficient tool for generating stable, sensitive T-lymphocyte lines to screen for GITR agonists.
The construct features constitutive expression of GITR and a drug selection marker (BSD), driven by the EF1a promoter, along with a dual reporter system comprising secreted Gaussia luciferase (GLuc) and GFP, driven by NF-κB response elements. This all-in-one high-titer purified lentivirus simplifies the process of establishing stable cell lines for functional assays.
This product is supplied as high-titer lentiviral particles (third generation), purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped, ideal for transducing difficult-to-transfect cells, including primary or thawed cells.
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Available Options:
Specifications
Details
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| TRV-0003 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| One vial | |
| 380 μl/vial | |
| 1-2×10^6 TU/vial, depending on the variant | |
| 3×10^8 VP/ml | |
| -80oC | |
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| Promoter | EF1α |
| Human GITR (C-terminal Flag) | |
| NM_004195 | |
| Puromycin (Puro) or Blasticidin (BSD) | |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| NFκB response elements | |
| GFP or RFP plus secreted Gaussia Luc (GLuc), separated by P2A | |
| T cell Activation. The product can be used to generate stable cell lines that read out GITR signaling through downstream activity of NFκB, for example in stable T or NK cell lines. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing T cell activity as a function of GITR stimulation/inhibition. |
*Based on infectivity on HEK293FT cells transduction units (TU).
Vector Layout
Advantages
- Versatile Readouts Luminescent and fluorescent dual readout provide unprecedented flexibility.
- Open Doors to Discovery Monitor signal pathway activity in vitro or in vivo with secreted GLuc.
- Innovative Technology Utilizes our novel reporter technology with upstream enhancer to boost signal intensity.
- License-free & Affordable Generate your own cell lines with ease, freeing you from constraints of expensive fixed commercial reporter cell lines. The EF1α promoter-driven receptor/ligand provides long-term stable expression in cell lines.
- Unmatched customization Various reporters and selection markers to choose from, perfectly tailored to your specific research needs.
Product Data
Figure 1. GITR/NFκB Reporter Fluorescence Validation HEK293FT cells were transfected with GITR/NFκB Reporter construct (EF1α-GITR-P2A-GFP, NFκB-RFP-GLuc). Constitutively expressed GITR and GFP are shown in the left and middle panels respectively, while RFP fluorescence in the right panels shows activation of the NFκB reporter in response to stimulation with human TNFα. The arrangement of components in this unique reporter system allow the user to select transduced cells by FACS sorting of GFP+ cells, whereas other variations of the product utilize a drug selection marker instead. Besides RFP expression, NFκB pathway activation can be examined also by luminescence detection of secreted Gaussia luciferase, depending on the user’s application or available instrumentation. This product is sold as high-titer prepackaged lentiviral particles, allowing the user to generate the cell line of their choosing. For more information, please email info@lipexogen.com.
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Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of potential customization options are shown in the table below and can be custom-made upon request. Please note that not all options may be feasible due to size limitations and this will depend on the specific product. We also welcome requests for custom products like the one shown on this page.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Lentiviral Transduction Protocol
MSDS
Frequently Asked Questions
Frequently Asked Questions
Can I purchase the plasmid for this product?
TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.