TCF/LEF (Wnt/Beta-Catenin) Reporter Cell Lines

Overview

Product Data:

Figure 1. TOPFlash EGFP Reporter Activation by LiCl in Stable HEK293 Cells

TOPFlash EGFP reporter (RCL-0011-5S) stable HEK293 cells were generated using TCF/LEF-TAG-BSD lentiviral particles and selected with blasticidin. Cells were seeded at 5 × 10⁴ cells per well in a 48-well plate and incubated for 8-10 hours. Cells were then treated with LiCl for 32 hours, trypsinized, and analyzed by flow cytometry to observe EGFP expression.

Figure 2. TOPFlash EGFP Reporter Activation by Human Wnt3a in Stable HEK293 Cells

TOPFlash EGFP reporter (RCL-0011-5S) stable HEK293 cells were seeded at 5 × 10⁴ cells per well in a 48-well plate and incubated for 8-10 hours. Cells were treated with 2.5 mM LiCl for 16 hours before addition of human Wnt3a to a final concentration of 25 ng/ml, then incubated for an additional 16 hours. Cells were then trypsinized and analyzed by flow cytometry.

Key Features:

• Reporter system:
  • Fluorescent enable real-time detection of TCF/LEF-mediated Wnt/Beta-Catenin signaling signaling in live cells.
• Stably integrated TCF/LEF responsive reporter construct reporter construct driving reporter expression.
• Validated with to confirm pathway responsiveness.
• Ideal for live-cell imaging, flow cytometry, or luminescence-based assays depending on format.

Applications:

• Screening activators and inhibitors of the TCF/LEF-mediated Wnt/Beta-Catenin signaling pathway.
• Studying transcriptional activity regulated by TCF/LEF-mediated Wnt/Beta-Catenin signaling in mammalian systems.
• Evaluating TCF/LEF-mediated transcriptional regulation.
• Fluorescence or luminescence-based readouts for high-throughput screening and mechanistic studies.

Quality Control: Each cell line is validated for reporter expression and pathway responsiveness using appropriate stimuli (e.g., ). Stable expression is confirmed for ≥20 passages. All cells are screened and certified mycoplasma-free.

Shipping and Storage: Shipped on dry ice (USA, Canada, Europe, Asia) Cells should be transferred to Liquid nitrogen vapor phase upon receipt.

How to Use: Plate cells and stimulate with the indicated pathway agonists or test compounds. Monitor reporter activity via fluorescence microscopy, flow cytometry, or luminescence detection depending on the construct.

Contents:

• 1 vial containing 5×10^6 cells TCF/LEF (Wnt/Beta-Catenin) HEK293 Reporter Cells (variant-specific).

Materials Required but Not Supplied:

• Media for Cell Culture:
  • Thaw Medium: DMEM, 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
  • Growth Medium: DMEM, 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
• Materials for Reporter Assays:
  • Pathway activators and/or inhibitors as needed (e.g., )
  • 96-well tissue culture-treated assay plates
  • Fluorescence microscope, flow cytometer, or luminometer (depending on reporter format)

Background:
TCF/LEF-mediated Wnt/Beta-Catenin signaling signaling plays a central role in cellular homeostasis, development, and disease. Activation of this pathway leads to modulation of TCF/LEF activity and downstream transcriptional responses. The reporter system used in these cells leverages defined response elements and a minimal promoter to drive reporter expression, enabling sensitive and specific measurement of pathway activation. These HEK293 reporter lines offer robust tools for dissecting pathway-driven transcriptional responses and screening modulators in live cells.

These products are covered by a Limited Use License. For more information, please email info@lipexogen.com