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h SIGLEC5 shRNA Lentivirus
$595.00 – $1,195.00
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h/m TSP1 shRNA Lentivirus
$595.00 – $1,195.00
h/m SERCA2 shRNA Lentivirus
$595.00 – $1,195.00
Validated human/mouse SERCA2 shRNA Lentivirus: High-titer lentivirus prepackaged with shRNA specific for human/mouse ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (SERCA2). The shRNA has been validated to meet or exceed 70% knockdown of SERCA2 using a specific fluorescence-based method that is more rapid and reliable than qPCR. The lentiviral particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, and are ideal for transducing difficult-to-transfect cells including thawed and/or primary cells. Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene (>70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs.
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Available Options:
Specifications
Product Data
Figure 1 (thumbnail). Knockdown validation for h/m SERCA2 shRNA Upper. HEK293FT cells were co-transfected with human SERCA2-V5 expression vector along with Scrambled-sh-GFP-Puro or h/m SERCA2 shRNAs. After 24-36 h, the cells were methanol fixed, stained for V5 tag, and fluorescence microscopy images were acquired. Lower. HaCaT cells were transduced with the corresponding viruses to obtain the stable cell lines. Western blot shows the protein levels of SERCA2 in the cells with scrambled shRNA or SERCA2 shRNA.
Figure 2. Knockdown validation for h/m SERCA2 shRNA HEK293FT cells were co-transfected with human SERCA2-V5 expression vector along with Scrambled-sh-GFP-Puro or h/m SERCA2 shRNAs (in our shRNA construct with the GFP reporter). After 24-36 h, the cells were methanol fixed, stained for V5 tag, and fluorescence microscopy images were acquired. GFP shows the cells that got the shRNA construct and Alexa-594 shows the expression of SERCA2 protein. Our shRNA lentivirus sets are made from a mixture of the 2 best performing shRNA constructs. Scr-sh, scrambled shRNA. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. cDNA expression vectors featured here are also available upon request. For more information, please email us at info@lipexogen.com.
Details
| LSV-0060 | |
| SERCA2 | |
| ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 | |
| Human/mouse |
Recommended Control
Scrambled shRNA Control Lentivirus (mixture of two independent shRNAs), LSV-0024
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA featured in the product figures are available upon request, supplied as plasmid or high-titer lentivirus.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
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Additional Information
Additional Information
- Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
- Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
- Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
- Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.
Vector Diagram
Vector Diagram
Size: 8.5 kb
The shRNA is driven by human U6 promoter. The EF1a promoter separately drives constitutive expression of a reporter gene and drug selection marker, separated by P2A.
Our Validation Process
Our Validation Process
