ORF cDNA Lentiviral Particles

Overview

These lentiviral particles enable efficient transduction and stable expression of the indicated ORF cDNA in mammalian cells. The vectors are optimized for high gene expression and consistent genome integration, supporting long-term cell line generation with reliable fluorescent (EGFP, mCherry) or luminescent (firefly luciferase) readouts.

Transcription of the C-terminally tagged ORF cDNA is driven by either the CMV or EF1α promoter. Reporter or drug selection markers (puromycin or blasticidin) are expressed separately using self-cleaving peptide sequences. In certain constructs, these markers are driven by the PGK promoter. The particles are suitable for transduction of various hard-to-transfect cell types, including primary and thawed cells. Cloning accuracy has been verified by sequencing, and expression has been confirmed via transient transfection.

Construct Design & Nomenclature

• Standard format: Promoter–ORFcDNA–Tag–T2A–Reporter–P2A–SelectionMarker
• Self-cleaving peptide shorthand: T2A and P2A sequences are denoted using slashes (“/”) in product names.Example: Gene-V5/Reporter/SelectionMarker represents Gene-V5–T2A–Reporter–P2A–SelectionMarker.
• Fusion constructs: A hyphen (“–”) denotes a direct fusion.Example: Gene-Reporter = Gene is fused directly to the reporter.
• All tags (e.g., Flag, V5, HA) are fused to the C-terminus of the ORF cDNA by default, unless stated otherwise.
• Abbreviations: GFP = EGFP; RFP = mCherry.
• For any product inquiries, contact us at info@lipexogen.com.

Key Advantages

• Optimized Vectors – LipExoGen ORF cDNA lentiviral particles are made using a third-generation system. These vectors have been extensively optimized for stable, high-level gene expression in mammalian cells with minimal toxicity. Ideal for primary or thawed cells. Constructs include a C-terminally tagged ORF driven by CMV or EF1α and may include one or two markers (GFP, RFP, firefly luciferase, puromycin, or blasticidin), separated by self-cleaving peptides. In alternative layouts, the PGK promoter drives marker expression separately.
• Versatile Readouts – C-terminal tags (e.g., V5, Myc, HA) allow for detection by tag-specific antibodies in fixed cells or lysates. In live cells, drug selection and FACS sorting using GFP or RFP enable rapid identification of transduced cells. GFP/RFP allows easy monitoring via microscopy or flow cytometry. Luciferase constructs can be used in in vivo studies with bioluminescence imaging (e.g., IVIS).
• Rapid Stable Line Generation – Particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. Suitable for primary and difficult-to-transfect cells. Stable lines can typically be established within 10 days using antibiotic selection or FACS.
• In Vivo Ready – Supplied in serum-free RPMI-1640. Vial size (~200–500 μl) ensures optimal stability for frozen storage. Easily concentrated with 10 kDa cutoff columns (Millipore-Sigma Cat# UFC510096) for stereotactic brain injection or other high-dose applications.
• Best Value & Customization – Every product is sequence-verified and expression-validated. Comparable or lower pricing than competitors. Custom modifications (e.g., promoter, tag, reporter, or selection marker changes) are available at no extra cost with 10-day turnaround. To request a modification or custom product, just contact us.

Product Details

*Based on infectivity on HEK293FT cells transduction units (TU).

Recommended Control

Empty Vector Ctrl ORF cDNA Lentivirus

Custom Orders

If you need to modify a product—such as changing the reporter or another vector component—please request a custom order. We offer fast, efficient custom cloning and high-quality lentiviral particle production, often at the same or lower price as catalog items.

If your gene of interest or transcript variant isn’t listed in our catalog, just let us know using one of the links below.

Quick Links

• Custom ORF cDNA Lentiviral Particles Request Form
• All Custom Lentiviral Particle Services