MEF2 Reporter Lentivirus

SKU: LTV-0068

$595.00

MEF2 Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse Myocyte enhancer factor 2 (MEF2) transcription factors in mammalian cells. Vertebrates have at least four versions of the MEF2 gene (MEF2A, MEF2B, MEF2C, and MEF2D), all expressed in distinct but overlapping patterns during embryogenesis through adulthood. MEF2 is a critical regulator of embryonic and heart development. MEF2 affects myocyte differentiation by regulating cardiac gene expression. In adult organisms, MEF2 proteins mediate the stress response in some tissues. The MEF2 Reporter Lentivirus contains tandem repeats of the consensus MEF2 DNA-binding elements to readout transcriptional activity of MEF2 transcription factors (e.g. MEF2A, MEF2B, MEF2C, MEF2D) in mammalian cells. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying MEF2 activity in difficult-to-transfect cells including primary and/or thawed cells.

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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.

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Specifications

Details

LTV-0068
MEF2 transcription factors
MEF2 consensus DNA-binding elements
Monitor transcriptional activity of Myocyte enhancer factor 2 (MEF2) transcription factors (TF) in mammalian cells, screen for activators or inhibitors of MEF2 TFs. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. MEF2 is a critical regulator in embryonic and heart development. MEF2 affects myocyte differentiation by regulating cardiac gene expression. In adult organisms, MEF2 proteins mediate the stress response in some tissues. The MEF2 Reporter Lentivirus contains tandem repeats of the consensus MEF2 DNA-binding elements to readout transcriptional activity of MEF2 transcription factors (e.g. MEF2A, MEF2B, MEF2C, MEF2D) in mammalian cells.
Human/mouse

 

 

Product Data

 

Figure 1. GFP reporter activation in cells co-transfected with MEF2-TAG-Puro reporter and MEF2A-P2A-RFP expression vector. HEK293FT cells were transfected with MEF2-TAG-Puro plasmid along with empty vector or an expression vector for human MEF2A with an RFP reporter separated by P2A sequence. After 24-36 h, images were acquired by fluorescence microscopy. GFP indicates the reporter activation, whereas RFP shows the cells that received the MEF2A cDNA vector. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. MEF2A cDNA expression vector is also available upon request. For more information, please email us.

 

 

 

 

 

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Recommended Controls

Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.

Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.

 

Custom Orders

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

ORF cDNA plasmids featured in the product figures are available upon request.

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Additional Information

Additional Information

  • High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
  • Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
  • Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
  • Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
  • Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
  • Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Vector Diagram

Vector Diagram

 

 

 

 

 

 

Size: 6.5-8.0 kb