miR-199a-5p Lentivirus: miRNA lentiviral particles for expression of human/mouse miR-199a (5p). The lentiviral vector contains the entire human miRNA stem-loop, which leads to expression of the human/mouse common mature miRNA sequence in transduced cells. The miRNA lentivirus is ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, ideal for in vitro or in vivo use and transduction of difficult-to-transfect (e.g. thawed and/or primary) cells.

Key Advantages:

• Superior vectors – LipExoGen miRNA Lentiviruses are produced using the third generation system and feature our own proprietary, high-quality vectors that have been optimized for high gene expression and reliable genome integration. All miRNA constructs contain the entire human miRNA stem-loop driven by U6 and PGK driven reporter plus drug selection marker separated by a self-cleaving peptide.
• Superior accuracy – Polyclonal miRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells.
• Easily identify transduced cells – Our miRNA constructs contain both fluorescent reporter and drug selection marker, allowing transduced cells to be selected by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase + GFP/RFP dual-reporters are also available upon request and can be used for detecting transduced cells in vitro or in vivo using luminescence-based techniques (for more details, contact us).
• Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of miRNA vectors, click here.