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NFκB-ASC (Pyroptosis) Reporter Lentivirus
$695.00 – $1,495.00
NFκB-ASC (Pyroptosis) Reporter is designed to monitor the formation of pyroptotic ASC specks in response to inflammasome activation. This system expresses GFP-tagged ASC, enabling visualization of pyroptotic ASC specks as markers of inflammasome complex formation. ASC polymerization is triggered by danger signals, forming specks that accumulate in the extracellular space. This reporter can be stably expressed in appropriate cells, such as THP-1 cells, and is useful for studying pyroptosis, a pro-inflammatory form of programmed cell death, in vitro.
This product is supplied as high-titer lentiviral particles (third generation), which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells, including primary or thawed cells.
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Available Options:
Specifications
Details
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| TRV-0037 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| One vial | |
| 380 μl/vial | |
| 3×10^6 TU/vial | |
| 3×10^8 VP/ml | |
| -80°C | |
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| Puromycin (Puro) | |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| NFκB response elements | |
| ASC-GFP fusion protein | |
| Pyroptosis assay to monitor ASC speck formation, essential for studying inflammasome activation. The product is ideal for analyzing NLRP3 inflammasome and other related inflammatory responses through GFP-tagged ASC and NFκB signaling. |
*Transduction units (TU), based on the number of drug resistant or fluorescent HEK293FT cells after transduction with the virus.
Background
Vector Layout
Product Data
Figure 1. THP-1 cells (5×10^5) were transduced with 50 µl lentivirus of NF-κB-ASC-GFP-Puro and spun for 2 hours. Cells were cultured for 72 hours and selected with 1.5-2 µg/ml puromycin for 7-10 days. They were then treated with 1 µg/ml LPS for 16 hours and 2.5 µg/ml polyIC mixed with Lipofectamine for 24 hours. The extracellular ASC specks indicate pyroptotic activation. Despite being a weak inducer, intracellular polyIC also led to pyroptotic signaling.
Advantages
- Versatile Readouts Fluorescent readout provide unprecedented flexibility.
- Innovative Technology Utilizes our novel reporter technology with upstream enhancer to boost signal intensity.
- License-free & Affordable Generate your own cell lines with ease, freeing you from constraints of expensive fixed commercial reporter cell lines.
- Unmatched customization Various reporters and selection markers to choose from, perfectly tailored to your specific research needs.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of potential customization options are shown in the table below and can be custom-made upon request. Please note that not all options may be feasible due to size limitations and this will depend on the specific product. We also welcome requests for custom products like the one shown on this page.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Lentiviral Transduction Protocol
MSDS
Frequently Asked Questions
Frequently Asked Questions
Can I purchase the plasmid for this product?
TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.