NFE2 Reporter Lentivirus

SKU: LTV-0069

$595.00

NFE2 Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse Nuclear factor, erythroid 2 (NFE2, 45 kDa subunit) activity in mammalian cells. NFE2 is essential for regulating erythroid and megakaryocytic maturation and differentiation, including platelet production, megakaryocyte biogenesis, and erythroid cell function. The NFE2 protein forms heterodimers with small MAF proteins such as MAF-G to regulate gene transcription. The NFE2 reporter lentivirus contains consensus NFE2 DNA-binding elements from the PBGD promoter to read out transcriptional activity of NFE2. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying NFE2 activity in difficult-to-transfect cells including primary and/or thawed cells.

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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.

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Specifications

Details

LTV-0069
NFE2 pathway
NFE2 DNA-binding elements (from the PBGD promoter)
Monitor NFE2 transcriptional activity in mammalian cells, screen for activators or inhibitors of NFE2 signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. NFE2 is essential for regulating erythroid and megakaryocytic maturation and differentiation, including platelet production, megakaryocyte biogenesis, and erythroid cell function. The NFE2 protein forms heterodimers with small MAF proteins such as MAF-G to regulate gene transcription. The NFE2 reporter lentivirus contains consensus NFE2 DNA-binding elements from the PBGD promoter to read out transcriptional activity of NFE2.
Human/mouse

 

 

Product Data

 

Figure 1. NFE2-TAG-Puro reporter activity in transiently transfected HEK293FT cells.   HEK293FT cells were co-transfected with NFE2-TAG-Puro and various expression vectors indicated in the figure. After 24-36 h, images were acquired by fluorescence microscopy. Top row: basal activation of the NFE2 reporter can be seen in HEK293FT cells co-transfected with empty vector (pcDNA). Second row: co-transfection with an expression vector for human MAF-G inhibits NFE2 reporter activity. MAF-G expression is indicated by RFP, which is separated from MAF-G by a P2A sequence. Third row: co-transfection with human NFE2 increases NFE2 reporter activity (RFP indicates the overexpression of NFE2). Bottom row: MAF-G inhibits NFE2 reporter activation from NFE2. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. MAF-G and NFE2 cDNA expression vectors are also available upon request. For more information, please email us.

 

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Recommended Controls

Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.

Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.

 

Custom Orders

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

ORF cDNA plasmids featured in the product figures are available upon request.

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Additional Information

Additional Information

  • High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
  • Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
  • Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
  • Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
  • Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
  • Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Vector Diagram

Vector Diagram

 

 

 

 

 

 

Size: 6.5-8.0 kb