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NFE2 Reporter Lentivirus
$595.00
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NFκB (p65) Reporter Lentivirus
$595.00
NFκB p65 Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse nuclear factor kappa B (NFκB) p65 (i.e. REL-A) homodimers in mammalian cells. The REL-A (p65) reporter lentivirus contains tandem repeats of the p65 DNA-binding element that allows for the discrimination of p65/p65 homodimer vs p50/p65 heterodimer transcriptional activity when used alongside the pan NFκB reporter (LTV-0002). The NFκB p65 reporter could be useful for studying non-canonical NFκB signaling, such as in tumor cells as opposed to inflammation. This reporter differs from the pan NFκB reporter which can detect both p50/p65 heterodimers induced by TNFα as well as p65/p65 homodimers. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying p65/p65 transcriptional activity in difficult-to-transfect cells including primary and/or thawed cells.
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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0070 | |
| NFκB Signaling Pathway | |
| NFκB p65 DNA-binding elements | |
| Monitor transcriptional activity of NFκB p65 homodimer in mammalian cells, screen for activators or inhibitors of NFκB p65 homodimer signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. The REL-A (p65) reporter lentivirus contains tandem repeats of a homodimer p65 DNA-binding element that allows for the discrimination of p65/p65 homodimer vs p50/p65 heterodimer transcriptional activity when used alongside the pan NFκB reporter (LTV-0002). The NFκB p65 reporter could be useful for studying non-canonical NFκB signaling mechanisms such as in tumor cells as opposed to inflammation. This reporter differs from the pan NFκB reporter which can detect both p50/p65 heterodimers induced by TNFα, as well as p6/p65 homodimers. | |
| Human/mouse |
Product Data
Figure 1. Comparison of NFκB (pan) and NFκB (p65/p65) reporter activity in response to TNFα and REL-A (p65) overexpression. HEK293FT cells were transfected (24-36 h) with the pan NFκB reporter (LTV-0002, top panels) or NFκB p65/p65 reporter (bottom panels). At the same time, some of the cells were co-transfected with empty vector (pcDNA, left) or an expression vector for human REL-A (p65, middle). After the transfection period of 24-36 h, cells on the right were stimulated with TNFα for 8 h, and then fluorescent images were acquired for all of the cells. Top panel shows the general activation of NFκB reporter by either TNFα or overexpression of p65, which is comparable. Bottom panels show the preferential activation of the p65 reporter activity in absence of TNFα and p65 overexpression, and minor activation by TNFα alone. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. REL-A (p65) cDNA expression vector is also available upon request. For more information, please email us.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Recommended Controls
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand, usually for the same or comparable price as the listed item.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
ORF cDNA plasmids featured in the product figures are available upon request.
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Additional Information
Additional Information
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by NFkB p65 homodimers are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb