Lentiviral ORF cDNA Expression Plasmids

Overview

These plasmids enable high-level expression of the indicated ORF cDNA in mammalian cells following transfection. Each vector is optimized for efficient gene expression and can be used to generate high-titer lentiviral particles when co-transfected with compatible packaging plasmids. These constructs support long-term applications such as stable cell line development and include options for fluorescent (e.g., EGFP, mCherry) or luminescent (e.g., firefly luciferase) reporters.

Transcription of the C-terminally tagged ORF cDNA is driven by either the CMV or EF1α promoter. Reporter or drug selection markers (puromycin or blasticidin) are expressed separately using self-cleaving peptide sequences. In certain constructs, these markers are driven by the PGK promoter. The particles are suitable for transduction of various hard-to-transfect cell types, including primary and thawed cells. Cloning accuracy has been verified by sequencing, and expression has been confirmed via transient transfection.

Construct Design & Nomenclature

• Standard format: Promoter–ORFcDNA–Tag–T2A–Reporter–P2A–SelectionMarker
• Self-cleaving peptide shorthand: T2A and P2A sequences are denoted using slashes (“/”) in product names. Example: Gene-V5/Reporter/SelectionMarker represents Gene-V5–T2A–Reporter–P2A–SelectionMarker.
• Fusion constructs: A hyphen (“–”) denotes a direct fusion. Example: Gene-Reporter = Gene is fused directly to the reporter.
• All tags (e.g., Flag, V5, HA) are fused to the C-terminus of the ORF cDNA by default, unless stated otherwise.
• Abbreviations: GFP = EGFP; RFP = mCherry.
• For any product inquiries, contact us at info@lipexogen.com.

Key Advantages

• Optimized Vectors – LipExoGen ORF cDNA expression plasmids are built on a third-generation lentiviral-compatible backbone. These vectors have been extensively optimized for stable, high-level gene expression in mammalian cells with minimal toxicity. Ideal for use in transfection workflows or lentiviral packaging. Constructs feature a C-terminally tagged ORF driven by CMV or EF1α and may include one or two markers (GFP, RFP, firefly luciferase, puromycin, or blasticidin), separated by self-cleaving peptides. Some layouts feature independent marker expression from a PGK promoter.
• Versatile Readouts – C-terminal tags (e.g., V5, Myc, HA) allow for detection by tag-specific antibodies in fixed cells or lysates. In live cells, drug selection and FACS sorting using GFP or RFP enable rapid identification of successfully transfected or transduced cells. Reporter fluorescence can be monitored via microscopy or flow cytometry. Luciferase constructs support sensitive quantification or in vivo imaging (e.g., IVIS) when packaged as virus.
• Flexible for Stable Line Generation – These plasmids are compatible with lentiviral packaging systems, enabling stable integration when needed. For transient studies, direct transfection yields robust expression. Stable cell lines can typically be generated within 10 days using antibiotic selection or FACS.
• Ready-to-Use Format – Supplied in PCR-grade water as high-purity, transfection-ready DNA. Each lot is thoroughly quality-tested for sequence integrity and yield, making it suitable for immediate use in transfection or lentiviral packaging. Ideal for fast-moving research workflows.
• Best Value & Customization – Every product is sequence-verified and expression-validated. Competitive pricing with free customization options, including promoter swaps, tag or reporter changes, and alternate marker configurations. Most custom requests are fulfilled within 10 days—just contact us.

Product Details

Recommended Control

Empty Vector Ctrl ORF cDNA Lentiviral Plasmid

Custom Orders

If you need to modify a product—such as changing the reporter or another vector component—please request a custom order. We offer fast, efficient custom cloning and high-quality lentiviral particle production, often at the same or lower price as catalog items.

If your gene of interest or transcript variant isn’t listed in our catalog, just let us know using one of the links below.

Quick Links

• Custom ORF cDNA Lentiviral Particles Request Form
• All Custom Lentiviral Particle Services