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HIF1A Wild Type & Mutant ORF cDNA Lentivirus
$695.00
Human and mouse hypoxia inducible factor 1 alpha (HIF1A/Hif1a) ORF cDNA lentivirus, available in both wild-type and normoxia-stable mutant (P402A/P577A/N813A) forms. Lentiviral particles are packaged with expression vectors encoding either the human or mouse HIF1A ORF cDNA. In mutant constructs, the ORF contains point mutations (P402A/P577A/N813A) that render the resulting HIF-1α protein resistant to oxygen-mediated degradation. Expression is driven by a CMV promoter, and the ORF is fused to a C-terminal V5 tag, and an eGFP reporter, separated by a self-cleaving peptide.
These lentiviral particles enable easy transduction and high-level stable expression of the specified ORF cDNA in mammalian cells. The lentiviral vectors are optimized for robust gene expression and stable genome integration, facilitating the establishment of long-term cell lines with consistent fluorescent (GFP, RFP) or luminescent (firefly luciferase) reporter output. ORF cDNA transcription is regulated by either EF1a or CMV promoter, while expression of the reporter or drug selection marker (puromycin or blasticidin) is linked via a self-cleaving peptide for bicistronic expression. In some constructs, the CMV or EF1a promoter drives expression of cDNA-Tag, while the GFP/RFP reporter and drug selection cassette are controlled by a separate PGK promoter. These particles efficiently transduce a broad range of cell types, including primary and previously frozen cells, offering a versatile tool for gene function studies in mammalian systems. ORF cDNA integrity has been verified by sequencing, and protein expression confirmed via transient transfection.
Available Options:
Specifications
Key Advantages:
- Optimized vectors – All LipExoGen ORF cDNA lentiviral particles are produced by the third generation system. They feature novel vectors that have been extensively optimized for high, stable gene expression in mammalian cells, with minimal toxicity, and are ideal for use in sensitive cells such as primary and/or thawed cells. CMV or EF1α drives expression of ORF cDNA fused to C-terminal epitope tag, followed by one or two reporter/drug selection markers (GFP, RFP, firefly Luc, puromycin, blasticidin). Each component is separated by self-cleaving peptides to allow independent protein expression from a single mRNA transcript. Alternatively, CMV or EF1α drives expression of C-terminal tagged ORF cDNA, and human or mouse PGK separately drives expression of reporter (GFP, RFP) and selection marker (puromycin, blasticidin), separated by P2A.
- Versatile readout of gene expression – ORF cDNA contains an epitope tag (e.g. V5, Myc, HA, etc.) fused to the C-terminus, which allows for tag-specific antibodies to be used for purification, or for staining in fixed cells or cell lysates. In live cells, puromycin or blasticidin allows stable cell line generation; transduced cells may also be isolated by FACS sorting based on GFP or RFP. GFP or RFP allows for convenient analysis of ORF cDNA expression by simple fluorescence microscopy, flow cytometry, or other common techniques, without unwanted effects of large fusion protein constructs. For in vivo studies, constructs with luciferase can be used to monitor cell growth by in vivo bioluminescence imaging techniques (e.g. IVIS).
- Easily establish stable cell lines expressing gene of interest – LipExoGen ORF cDNA lentiviral particles are ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and are suitable for transduction of primary cells, stem cells, and difficult-to-transfect cells including primary or thawed cells. Transduced cells can be FACS sorted by GFP or RFP, or selected by puromycin or blasticidin, to yield stable cells in about 10 days.
- Multiple applications – LipExoGen ORF cDNA lentiviral particles are supplied in serum-free cell culture medium allowing them to be directly applied in vivo. For optimal stability of the lentivirus in frozen storage, the particles are provided at an ideal concentration (about 200-500 µl per aliquot), but they can be easily concentrated with 10 kDa cutoff columns (Millipore-Sigma Cat# UFC510096) for in vivo use where higher concentrations are desirable, such as for stereotactic brain injections.
- Best value & easy customization – All ORF cDNA lentiviral vectors are expression validated and sequence verified. Together with the previously mentioned advantages and a price that is comparable or less vs the competition, LipExoGen ORF cDNA lentiviral particles are the best way to express and study your gene of interest in human or mouse cells. And, if you don’t find what you’re looking for in our catalog, you can easily customize the promoter, tag, reporter, or selection marker of one of our listed ORF cDNA lentivirus and receive the same quality lentivirus without additional cost in about 10 working days. To modify components of a listed ORF cDNA lentivirus, or to request a custom ORF cDNA lentivirus or plasmid based on your specifications, simply contact us.
Details:
| LCV-0001 | |
| hypoxia inducible factor 1, alpha subunit | |
| ORF cDNA Lentiviral Particles |
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Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please request a custom order. We provide a variety of fast and efficient services for custom cloning or the production of high-quality custom lentiviral particles on demand, usually for the same or comparable price as the listed item. If you’re unable to find your ORF cDNA of interest in our catalog, or you require a different transcript variant than the one listed, please send us a request using the link below.
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Additional Information
Additional Information
| Hif1a | |
| hypoxia inducible factor 1, alpha subunit | |
| n | |
| Mus musculus | |
| Alias | HIF1A; hypoxia inducible factor 1 subunit alpha; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia-Inducible Factor 1-Alpha; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; BHLHe78; PASD8; MOP1; Hypoxia-Inducible Factor 1 Alpha Isoform I.3; Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; ARNT-Interacting Protein; HIF-1alpha; HIF1-ALPHA; HIF1-Alpha; BHLHE78; HIF-1A; HIF1 |
| Annotation Page | |
| Gene IDs | MGI:MGI:106918 Ensembl:ENSMUSG00000021109 |
| Entrez Gene Summary | “This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]” |