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Lentiviral Promoter Reporter Kit
$95.00 – $895.00
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CD16A/NFAT Reporter Lentivirus (ADCC Assay)
$895.00 – $1,695.00
PD1-CD28/NFAT Reporter Lentivirus
$895.00
The PD1-CD28/NFAT Immunotherapy Reporter is designed to measure T cell activity in response to modulation of the PD-1 pathway. The lentiviral construct utilizes EF1a promoter to drive constitutive expression of human PD1 and CD28, which are separated by a P2A linker, resulting in the expression of two separate proteins in the transduced cells. Downstream of CD28 is a drug selection marker or fluorescent reporter gene which is also constitutively expressed as a separate protein, allowing the user to select drug resistant or fluorescent cells for easy generation of stable cell lines. A separate expression cassette within the same construct utilizes tandem repeats of NFAT response elements coupled to a minimal TATA promoter and upstream proprietary enhancer element to drive expression of the reporter gene(s), which include a fluorescent reporter (GFP or RFP) and/or Gaussia Luciferase (GLuc), providing a convenient approach to read out T cell activity. This product is supplied as high-titer lentiviral particles, which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells including primary or thawed cells.
Have questions about this product? Need a stable cell line? Contact Us
Available Options:
Specifications
Details
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| TRV-0005 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| One vial | |
| 380 μl/vial | |
| 1-2×10^6 TU/vial, depending on the variant | |
| 3×10^8 VP/ml | |
| -80oC | |
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| Promoter | EF1α |
| Human PD1 and CD28, separated by P2A (C-terminal Flag) | |
| NM_0050183 and NM_006139 | |
| Puromycin (Puro) or Blasticidin (BSD) | |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| NFAT response elements | |
| GFP or RFP plus secreted Gaussia Luc (GLuc), separated by P2A | |
| Immune checkpoint inhibitor assay, T cell activation assay. The product can be used to generate stable cell lines that read out PD1/CD28 signaling through downstream activity of NFAT, for example in stable T cell lines. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing T cell activity as a function of PD1/CD28 stimulation/inhibition. |
*Transduction units (TU), based on the number of drug resistant or fluorescent HEK293FT cells after transduction with the virus.
Vector Layout
Advantages
- Versatile Readouts Luminescent and fluorescent dual readout provide unprecedented flexibility.
- Open Doors to Discovery Monitor signal pathway activity in vitro or in vivo with secreted GLuc.
- Innovative Technology Utilizes our novel reporter technology with upstream enhancer to boost signal intensity.
- License-free & Affordable Generate your own cell lines with ease, freeing you from constraints of expensive fixed commercial reporter cell lines. The EF1α promoter-driven receptor/ligand provides long-term stable expression in cell lines.
- Unmatched customization Various reporters and selection markers to choose from, perfectly tailored to your specific research needs.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of potential customization options are shown in the table below and can be custom-made upon request. Please note that not all options may be feasible due to size limitations and this will depend on the specific product. We also welcome requests for custom products like the one shown on this page.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Lentiviral Transduction Protocol
MSDS
Frequently Asked Questions
Frequently Asked Questions
Can I purchase the plasmid for this product?
TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.