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ATF4 Reporter Lentivirus
$595.00
RXRE/DR1 Reporter Lentivirus
$595.00
Fluorescent RXRE/DR1 Reporter Lentivirus: High-quality retinoid X receptor (RXR) response element (RXRE) lentiviral reporter system that provides a sensitive fluorescent (GFP or RFP) or luminescent (firefly Luc) readout for RXR transcriptional activity in mammalian cells. The RXRE/DR1 reporter contains tandem repeats of the consensus DR1 DNA binding element which is activated by RXR/RXR homodimer and suppressed by RXR/RAR heterodimer in response to 9-cis retinoic acid. RXRE/DR1 reporter can be used to study retinoic acid signaling pathway and detect transcriptional activities mediated by RXR. The lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for use with difficult-to-transfect cells including primary and/or thawed cells. All versions of this product feature a drug selection marker (puro/BSD) to facilitate easy establishment of stable cell lines.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0047 | |
| Retinoic acid signaling pathway, Retinoid X Receptor signaling | |
| Retinoid X receptor response element (RXRE) | |
| Monitor retinoic acid signaling pathway activation and the transcriptional activity mediated by RXR in mammalian cells. Screen for activators/inhibitors of RXRα and RXR signaling pathway. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Product Data
Figure 1. GFP reporter activation in cells co-transfected with RXRE/DR1-TAG-Puro and RXRα expression vector HEK293FT cells were co-transfected with the indicated plasmids for 24 h. All cells were subsequently treated with 25 µM retinoic acid (RA) for 8 h, followed image acquisition. Green shows the activation of the RXRE-TAG-Puro reporter (GFP), whereas RFP from the second plasmid indicates the cells with expression of human RXRα.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
- High Sensitivity – LipExoGen RXRE/DR1 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcriptional response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb