h/m BAX shRNA Lentivirus

Key Advantages:

• Same Cost For Custom Lentivirus – You can receive any combination of reporter (GFP/RFP/Luc/None) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of shRNA vectors, click here.
• Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
• Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
• Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
• Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.

Background

BCL2 associated X, apoptosis regulator (BAX) is a proapoptotic BCL-2 family member which participates in the regulation of apoptosis mediated by the intrinsic or mitochondrial pathway. The BAX gene encodes a 21 kDa protein known as BAX-alpha which, together with BCL2 antagonist/killer 1 (BAK1 or BAK), represent the major effectors of apoptosis by mediating mitochondrial outer membrane permeability (MOMP). In the intrinsic apoptosis pathway, MOMP results in release of soluble proteins including cytochrome c into the cytosol from the mitochondrial intermembrane space, which promotes the activation of cytosolic caspases. BAX-alpha mediates MOMP by translocating from the cytosol to the OMM, where it oligomerizes (1). In vertebrates, cells deficient in both BAX and BAK are resistant to MOMP and apoptosis in response to various death stimuli (2). The specific shRNA construct in BAX-sh-GFP-Puro lentiviral particles can efficiently knockdown human or mouse BAX expression (Fig. 1), making it useful for the study of BAX function and the intrinsic apoptosis pathway in human/mouse cells.

Product Data

Figure 1. Knockdown validation for BAX-sh2-GFP-Puro.   HEK293FT cells were co-transfected with human BAX-RFP bicistronic expression plasmid (red) and plasmids for the indicated shRNAs (GFP, green). After 24-36 hours, fluorescent images of the living cells were acquired by fluorescence microscopy. GFP represents transfection of the shRNA construct, whereas RFP indicates translation of the target mRNA. Higher knockdown efficiencies may be possible in stably-transfected cells. Sr-sh, scrambled shRNA. BAXα cDNA featured here is also available upon request: Click here to contact us

Figure 2. Knockdown validation for BAX-sh3-GFP-Puro.   HEK293FT cells were co-transfected with human BAX-RFP bicistronic expression plasmid (red) and plasmids for the indicated shRNAs (GFP, green). After 24-36 hours, fluorescent images of the living cells were acquired by fluorescence microscopy. GFP represents transfection of the shRNA construct, whereas RFP indicates translation of the target mRNA. Higher knockdown efficiencies may be possible in stably-transfected cells. Sr-sh, scrambled shRNA. Our “set” contains sequences sh2 and sh3. BAXα cDNA featured here is also available upon request: Click here to contact us

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Custom Orders

If you require a modification to one of our products (for example, change in reporter or other vector component), please request a custom order. We provide a variety of fast and efficient services for the production of high-quality, custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable shRNA cell line for you using this product. Learn more.

The cDNA lentivirus corresponding to this product is also available upon request, comparable price.

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