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CD32A/NFAT Reporter Lentivirus (ADCP Assay)
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STING/ISRE Reporter Lentivirus
$895.00
SIRPA/NFκB Reporter Lentivirus Set
$1,200.00
The SIRPA/NFκB Reporter Lentivirus is designed to study the function and signaling of SIRPA, a receptor that interacts with CD47 to inhibit phagocytosis, making it applicable for “Don’t Eat Me” Assay (Phagocytosis Inhibition) research. The construct constitutively expresses SIRPA, which upon engagement activates NFκB signaling, driving the expression of dual reporters: secreted Gaussia luciferase (GLuc) and GFP. These reporters enable quantification of NFκB activation and visualization of pathway-specific effects in real time. To use this system, transduce your desired cells with the high-titer purified lentivirus to establish a stable cell line for immune checkpoint studies. This product is supplied as high-titer lentiviral particles (third generation), purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped, ideal for difficult-to-transfect cells, including primary or thawed cells.
CD47, a cell surface molecule in the immunoglobulin superfamily, is expressed on both normal and tumor cells and interacts with several proteins in addition to SIRPα on macrophages and dendritic cells (DCs). The SIRPα/NF-κB reporter lentivirus is an effective tool for generating stable cell lines to measure the potency of SIRPα blocking antibodies or SIRPα-Fc in counteracting tumor evasion from macrophage-mediated killing.
This product features a dual reporter system comprising secreted Gaussia luciferase (GLuc) and GFP, driven by NF-κB response elements, and is designed for high-efficiency transduction and stable expression in your cells of interest. The high-titer purified lentivirus facilitates the generation of robust cell lines for functional assays.
This product is supplied as high-titer lentiviral particles (third generation), purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped, ideal for transducing difficult-to-transfect cells, including primary or thawed cells.
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Available Options:
Specifications
SIRPA/NFκB Reporter Lentivirus (Don’t Eat Me Assay) Details
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| TRV-0007 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| Set of two vials | |
| 380 μl/vial | |
| 2×10^6 TU/vial | |
| 3×10^8 VP/ml | |
| -80oC | |
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| Promoter | CMV |
| Human SIRPA (C-terminal Flag) | |
| NM_001040022 | |
| Puromycin (Puro) and Blasticidin (BSD) | |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| NFκB response elements | |
| eGFP or mCherry plus secreted Gaussia Luc (GLuc), separated by P2A | |
| ‘Don’t Eat Me’ assay. The product can be used to generate stable cell lines that read out SIRPA signaling through downstream activity of NFκB. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing cell activity as a function of SIRPA stimulation/inhibition. |
*Transduction units (TU), based on the number of drug resistant or fluorescent HEK293FT cells after transduction with the virus.
Vector Layout
Advantages
- Versatile Readouts Luminescent and fluorescent dual readout provide unprecedented flexibility.
- Open Doors to Discovery Monitor signal pathway activity in vitro or in vivo with secreted GLuc.
- Innovative Technology Utilizes our novel reporter technology with upstream enhancer to boost signal intensity.
- License-free & Affordable Generate your own cell lines with ease, freeing you from constraints of expensive fixed commercial reporter cell lines. The CMV promoter-driven receptor provides long-term stable expression in cell lines.
- Unmatched customization Various reporters and selection markers to choose from, perfectly tailored to your specific research needs.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of potential customization options are shown in the table below and can be custom-made upon request. Please note that not all options may be feasible due to size limitations and this will depend on the specific product. We also welcome requests for custom products like the one shown on this page.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Lentiviral Transduction Protocol
MSDS
Frequently Asked Questions
Frequently Asked Questions
Can I purchase the plasmid for this product?
TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.