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MyoD Reporter Lentivirus
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XRE Reporter Lentivirus
$595.00
T3RE/DR4 Reporter Lentivirus
$595.00
Fluorescent T3RE/DR4 Reporter Lentivirus: High-quality thyroid hormone receptor response element (T3RE/DR4) lentiviral reporter system that provides a sensitive readout (GFP, RFP, firefly Luc) for thyroid stimulating hormone receptor (TSHR) transcriptional activity in mammalian cells. The reporter construct contains tandem repeats of the consensus DR4 DNA binding element which is activated by TSHR in response to thyroid hormone T3 stimulation. An optimized mini-enhancer amplifies transcriptional activity mediated by T3RE/DR4 bound transcriptional factors to enable sensitive readout of TSHR pathway activity. The product is supplied as high-titer lentiviral particles featuring your choice of GFP (TAG), RFP (TAR) or firefly Luc (TAL) reporter, and all versions come with a drug selection marker (puro or BSD) for easy establishment of stable cell lines. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for use with difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0050 | |
| Thyroid stimulating hormone receptor (TSHR) | |
| Thyroid hormone response element (T3RE/DR4) | |
| Monitor TSHR activity in mammalian cells, screen for activators or inhibitors of TSHR. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Product Data
Figure 1 (thumbnail). HEK293FT cells were co-transfected for 24 h with plasmids for T3RE-TAG-Puro plus either empty vector or expression vector for human TSHR. The cells were then stimulated for 16 h with thyroid hormone T3 (30 nM) followed by image acquisition.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
- High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb