Tet-On FOXO3a ORF cDNA Lentivirus

Additional Information

Key Advantages:

• Optimized vectors – All LipExoGen ORF cDNA lentiviral particles are produced by the third generation system. They feature novel vectors that have been extensively optimized for high, stable gene expression in mammalian cells, with minimal toxicity, and are ideal for use in sensitive cells such as primary and/or thawed cells. CMV or EF1α drives expression of ORF cDNA fused to C-terminal epitope tag, followed by one or two reporter/drug selection markers (GFP, RFP, firefly Luc, puromycin, blasticidin). Each component is separated by self-cleaving peptides to allow independent protein expression from a single mRNA transcript. Alternatively, CMV or EF1α drives expression of C-terminal tagged ORF cDNA, and human or mouse PGK separately drives expression of reporter (GFP, RFP) and selection marker (puromycin, blasticidin), separated by P2A.
• Versatile readout of gene expression – ORF cDNA contains an epitope tag (e.g. V5, Myc, HA, etc.) fused to the C-terminus, which allows for tag-specific antibodies to be used for purification, or for staining in fixed cells or cell lysates. In live cells, puromycin or blasticidin allows stable cell line generation; transduced cells may also be isolated by FACS sorting based on GFP or RFP. GFP or RFP allows for convenient analysis of ORF cDNA expression by simple fluorescence microscopy, flow cytometry, or other common techniques, without unwanted effects of large fusion protein constructs. For in vivo studies, constructs with luciferase can be used to monitor cell growth by in vivo bioluminescence imaging techniques (e.g. IVIS).
• Easily establish stable cell lines expressing gene of interest – LipExoGen ORF cDNA lentiviral particles are ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and are suitable for transduction of primary cells, stem cells, and difficult-to-transfect cells including primary or thawed cells, and non-dividing cells. Transduced cells can be FACS sorted by GFP or RFP, or selected by puromycin or blasticidin, to yield stable cells in about 10 days.
• Multiple applications – LipExoGen ORF cDNA lentiviral particles are supplied in serum-free cell culture medium allowing them to be directly applied in vivo. For optimal stability of the lentivirus in frozen storage, the particles are provided at an ideal concentration (about 200-500 µl per aliquot), but they can be easily concentrated with 10 kDa cutoff columns (Millipore-Sigma Cat# UFC510096) for in vivo use where higher concentrations are desirable, such as for stereotactic brain injections.
• Best value & easy customization – All ORF cDNA lentiviral vectors are expression validated and sequence verified, providing a simple path to express and study your gene of interest in human or mouse cells. You can easily customize the promoter, tag, reporter, or selection marker of one of our listed ORF cDNA lentivirus by contacting us.