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ISRE Reporter Lentivirus
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GRE Reporter Lentivirus
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GAS Reporter Lentivirus
$595.00
Fluorescent GAS Reporter Lentivirus (IFNγ/JAK/STAT1 Pathway): High-quality lentiviral reporter system that provides fluorescent readout in response to interferon gamma-activated sites (GAS) transcriptional activation in human/mouse cells, useful for monitoring interferon gamma-induced signal transduction (i.e. IFNγ/JAK/STAT1) pathway activity. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying IFNγ/JAK/STAT1 pathway activity in difficult-to-transfect cells including primary and/or thawed cells.
Our PhD-level staff is ready to assist you. Email us to discuss your project or obtain help selecting the right product for your research.
Email: info@lipexogen.com
Available Options:
Specifications
Key Advantages:
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system and have been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Product Data:
Figure 1 (thumbnail). HEK293FT cells were transfected with GAS-TAG-Puro for 24 hrs, then stimulated with IFNγ or PBS for 12 hrs. GFP reporter activation was then visualized by fluorescence microscopy of the living cells.
Figure 2 (thumbnail). HEK293FT cells were transfected with GAS-TAR-BSD for 24 h, then stimulated with 10 ng/ml human IFNγ (DMEM, 10% FBS) for 16 h before acquiring images.
Have questions about this product? Need a custom modification or stable cell line? Email us and we will respond the same day.
Email: info@lipexogen.com
Details:
| LTV-0022 | |
| IFNγ/JAK/STAT1 pathway | |
| Interferon gamma-activated sites (GAS) | |
| Monitor interferon gamma-induced signal transduction pathway activity by detecting the binding of activated STAT1 homodimers to GAS. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).