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AARE Reporter Lentivirus
$595.00
Fluorescent AARE Reporter Lentivirus: High-quality, amino acid response element (AARE) lentiviral reporter system that provides a sensitive fluorescent readout to AARE/ATF4 activity (amino acid response pathway, eIF2α/ATF4 pathway) in human/mouse cells. This product uses tandem repeats of AARE from the human asparagine synthase promoter, which contain the nutrient-sensing response elements (NSRE-1 and NSRE-2), to drive the reporter expression in mammalian cells in response to amino acid or glucose deprivation. Transient (short-term) amino acid limitation activates GCN2 kinase, which phosphorylates eIF2a, resulting in ATF4 activation. Prolonged amino acid or glucose deprivation also makes the AARE respond to ER stress pathway activation. The transcription factor (TF) reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying amino acid response pathway activity/cellular response to metabolic stress in difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Key Advantages:
- High Sensitivity – LipExoGen AARE Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The amino acid response elements (AARE) are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Product Data:
Figure 1 (Thumbnail). HEK293 cells were transfected with AARE-TAG-Puro for ~24 hours, followed by ~12 hour treatment with thapsigargin (1 µg/ml, in 0.2% FBS DMEM), and GFP reporter activity was visualized by fluorescence microscopy.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Details:
| LTV-0037 | |
| Amino acid response pathway (eIF2α/ATF4 pathway) | |
| Amino acid response element (AARE) | |
| Monitor eIF2α/ATF4 pathway and amino acid/metabolic stress response in mammalian cells, screen for activators or inhibitors of the eIF2α/ATF4 pathway and amino acid/metabolic stress response. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Related Products
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb