c-MYC Reporter Lentivirus
Fluorescent c-MYC Reporter Lentivirus: High-quality, lentiviral reporter system that provides a sensitive fluorescent readout for human/mouse MYC proto-oncogene, bHLH transcription factor (c-MYC) activity in transduced cells. The transcription factor (TF) reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying c-MYC activity in difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Figure 1 (thumbnail). HEK293FT cells were co-transfected with c-MYC-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for human MYC (MYC, right). Fluorescence microscopy images were taken 24 hrs later to assess GFP reporter expression in the live cells.
Figure 2. GFP reporter activation in cells co-transfected with c-MYC-TAG-Puro and MYC-P2A-RFP. HEK293FT cells were co-transfected with MYC-TAG-Puro and either empty vector RFP (top) or human c-MYC expression plasmid (MYC-P2A-RFP). After ~24 hours, the living cells were examined by fluorescence microscopy to visualize GFP reporter activation of MYC-TAG-Puro construct in cells with concomitant human c-MYC over-expression (RFP).
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
|MYC proto-oncogene, bHLH transcription factor
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
The c-MYC oncogene is regarded as a master transcription factor for the regulation of cell growth and metabolism. In normal cells, developmental or mitogenic signals tightly regulate its expression and function. However, in cancer, c-MYC is frequently dysregulated (overexpressed) due to either gene amplification or as a result of other oncogenes which upregulate its expression. In transformed cells, c-MYC is known to upregulate target genes cyclin A2, cyklin D2, cyclin E1, serine hydroxymethyl transferase, enolase, LDH-A, EIF4E, HMG1/Y, nucleolin, NM23, and ribosomal proteins L3, L6, and S15A, among others. c-MYC also downregulates some genes including p21CIP1, p21INK4B, integrins and N-cadherin1. Thus, c-MYC is implicated in a diverse array of cellular pathways including growth factor response, C1 and iron metabolism, glycolysis, initiation of translation, cell transformation, proliferation, the DNA damage response, cellular adhesion, and the TGF-β pathway1. Although c-MYC is an attractive target for cancer therapy, direct specific targeting of transcription factor activities is challenging. As a result, the c-MYC reporter lentivirus can be used as a valuable tool for studying the transcriptional activity of c-MYC, identifying c-MYC activity in transduced cells, or for screening for novel c-MYC inhibitors, etc. The vector construct has been validated to provide a sensitive fluorescent readout in response to c-MYC transcriptional activity (Fig. 1).
|MYC proto-oncogene, bHLH transcription factor
|Homo sapiens/mus musculus
|MYC Proto-Oncogene, BHLH Transcription Factor; V-Myc Avian Myelocytomatosis Viral Oncogene Homolog; Class E Basic Helix-Loop-Helix Protein 39; Myc Proto-Oncogene Protein; Transcription Factor P64; Proto-Oncogene C-Myc; BHLHe39; Myc-Related Translation/Localization Regulatory Factor; Avian Myelocytomatosis Viral Oncogene Homolog; V-Myc Myelocytomatosis Viral Oncogene Homolog; BHLHE39; C-Myc; MRTL; MYCC
|HGNC:HGNC:7553 Ensembl:ENSG00000136997 MIM:190080
|Entrez Gene Summary
|“This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]“