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FOXO1/IRE Reporter Lentivirus
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MRE Reporter Lentivirus
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FOXO3 Reporter Lentivirus
$595.00 – $695.00
Fluorescent FOXO3 Reporter Lentivirus: High-quality lentiviral reporter system that provides a sensitive fluorescent readout to FOXO3 activity in human/mouse cells. The transcription factor (TF) reporter is preferentially activated by FOXO3 (vs FOXO1) and can readout transcriptional inhibition by FOXP1. The lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for use with difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0041 | |
| Forkhead transcription factors | |
| FOXO3 | |
| Monitor FOXO3 activity in mammalian cells, screen for activators or inhibitors of FOXO3. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. Preferentially activated by FOXO3 (vs FOXO1). FOXO3-induced activation is inhibited by FOXP1. | |
| Human/mouse |
Product Data
Figure 1. GFP reporter activation in cells co-transfected with FOXO3-TAG-Puro and different forkhead TFs. HEK293FT cells were co-transfected with FOXO3-TAG-Puro and either empty vector or the forkhead TFs annotated in the figure. After 24-36 hours, the living cells were examined by fluorescence microscopy to visualize GFP reporter activity. FOXO3-TAG-Puro is preferentially activated by FOXO3, with minor activation by FOXO1. FOXO3-induced activation is inhibited by FOXP1.
Figure 2. β-Lactamase reporter activation in cells co-transfected with cDNA encoding human FOXO3 HEK293FT cells were co-transfected with FOXO3-β-Lactamase-V5-BSD plus FOXO3a-Myc or pcDNA plasmids for 32 h. After that, the cells were methanol fixed and stained with Alexa 488 conjugated anti-V5 antibody to detect β-Lactamase expression.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
- High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb