GAL4 Reporter Lentivirus

SKU: LTV-0038

$595.00

Fluorescent GAL4 Reporter Lentivirus: High-quality GAL4 lentiviral reporter system useful for the rapid readout of protein-protein interactions (PPI), suitable as a reporter for mammalian two-hybrid assays. The construct contains 5 tandem repeats of UAS GAL4 upstream of fluorescent (GFP or RFP) reporter, which allows convenient readout of reporter activity by fluorescence microscopy or flow cytometry.  Fluorescence is produced in response to GAL4 DBD fusion protein and is greatly enhanced by VP16 fusion protein if the two variable domains interact. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for transducing difficult-to-transfect cells including primary and/or thawed cells. If you want to clone component/domain for PPI or mammalian two-hybrid assay, please see our Gene Regulation Custom Service .

You can easily request custom made fusion protein plasmids (e.g. GAL4-X and Y-VP16) by contacting us. 

Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us

Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.

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Specifications

Details

LTV-0038
GAL4
Identification of protein-protein interactions (PPI) and as a reporter in mammalian two-hybrid assay
Human/mouse

 

 

Product Data

 

Figure 1. GAL4 reporter activity in response to HIF1a-CAD alone, or with binding partner P300-CH1-VP16. HEK293FT cells were co-transfected with GAL4-TAG-Puro along with the following construct(s): P300-CH1-VP16 (left), GAL4-HIF1a-CAD (middle), or GAL4-HIF1a-CAD + P300-CH1-VP16 (right). Images were acquired 24-36 h later. The GAL4 DNA binding domain (DBD) fusion protein activates the GAL4-TAG-Puro reporter, which contains 5 tandem repeats of UAS GAL4-DNA binding elements upstream of GFP. The example shows the reporter activation in response to GAL4 fused to HIF-1 alpha C-terminal activation domain (CAD) (middle). Reporter activity is substantially enhanced in the presence of VP16 co-activator fused to P300-CH1 because of protein-protein interaction (PPI) between HIF1a-CAD and P300-CH1 (right). VP16 alone cannot activate the reporter (left). The system can be used to identify PPI or as a reporter in mammalian two-hybrid assays.

 

Have questions about this product? Send us a form and we’ll reply the same day: Contact Us

 

Recommended Controls

Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.

Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.

 

Custom Orders

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

Plasmids for fusion proteins featured in the product figures are available upon request. We will also gladly create custom fusion constructs for your studies upon request.

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Gene Regulation Services

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Premade TF/Signal Pathway Reporter Lentivirus

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Additional Information

Additional Information

  • High Sensitivity – LipExoGen GAL4 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The GAL4-DNA binding response elements are arranged as 5 tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
  • Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
  • Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
  • Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
  • Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
  • Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Vector Diagram

Vector Diagram

 

 

 

 

 

 

Size: 6.5-8.0 kb