NOTCH Reporter Lentivirus
Fluorescent NOTCH Reporter Lentivirus: High-quality lentiviral reporter system that provides fluorescent readout in response to human/mouse constitutive NOTCH intracellular domain (NICD, or cNOTCH), useful for the sensitive detection of Notch signaling pathway activation. The transcription factor reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying Notch activity in difficult-to-transfect cells including primary and/or thawed cells.
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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system and have been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Figure 1 (thumbnail). HEK293FT cells were co-transfected with NOTCH-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for human constitutively active NOTCH1 intracellular domain (cNOTCH1). Fluorescence microscopy images were taken 24-36 hrs later to assess GFP reporter expression in the live cells.
Figure 2. Comparison of NOTCH1 TAG and d2G reporter plasmids in co-transfection with NOTCH1 NICD. HEK293FT cells were co-transfected with the indicated plasmids for 24-36hrs. Compared to the traditional GFP reporter (TAG), degradable d2GFP (d2G) shows slightly reduced background and GFP intensity in the cells with overexpressed NOTCH NICD. The degradable reporter could potentially be useful in studying temporal activity of the NOTCH pathway. NOTCH1 NICD expression vector (plasmid) can be purchased by clicking here: NOTCH1 NICD cDNA Plasmids
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
|NOTCH responsive element|
|Monitor NOTCH pathway activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.|
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
NOTCH1 NICD Positive Control Expression Vector (Plasmid) – Can be used as a positive control for NOTCH1 reporter activation in permissive cells, may be useful for troubleshooting to establish ideal extracellular stimulation conditions for NOTCH pathway activation (i.e. beads, ligand, etc.)
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
|notch receptor 1|
|Homo sapiens/mus musculus|
|Alias||Notch Receptor 1; Notch 1; Translocation-Associated Notch Protein TAN-1; Neurogenic Locus Notch Homolog Protein 1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1, Translocation-Associated (Drosophila); Notch Homolog 1, Translocation-Associated; EC 18.104.22.168; EC 22.214.171.124; AOVD1; AOS5|
|Gene IDs||HGNC:HGNC:7881 Ensembl:ENSG00000148400 MIM:190198|
|Entrez Gene Summary||“This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]“|