NOTCH Reporter Lentivirus

Key Advantages:

• High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
• Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
• Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
• Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
• Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system and have been validated to read out the indicated transcription factor activity.
• Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Product Data:

Figure 1 (thumbnail). HEK293FT cells were co-transfected with NOTCH-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for human constitutively active NOTCH1 intracellular domain (cNOTCH1). Fluorescence microscopy images were taken 24-36 hrs later to assess GFP reporter expression in the live cells.

Figure 2. Comparison of NOTCH1 TAG and d2G reporter plasmids in co-transfection with NOTCH1 NICD. HEK293FT cells were co-transfected with the indicated plasmids for 24-36hrs. Compared to the traditional GFP reporter (TAG), degradable d2GFP (d2G) shows slightly reduced background and GFP intensity in the cells with overexpressed NOTCH NICD. The degradable reporter could potentially be useful in studying temporal activity of the NOTCH pathway. NOTCH1 NICD expression vector (plasmid) can be purchased by clicking here: NOTCH1 NICD cDNA Plasmids

Details:

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

Quick Links:

Custom Lentivirus Services

Premade TF/Signal Pathway Reporter Lentivirus

All Lentivirus Products