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P53 Reporter Lentivirus
$595.00
Fluorescent P53 Reporter Lentivirus (P53/Apoptosis Pathway): High-quality, fluorescent lentiviral transcription factor (TF) reporter system that provides sensitive fluorescent readout for human/mouse tumor protein p53 activity in transduced cells. P53 drives expression of the fluorescent reporter, making the product useful for the sensitive detection of apoptosis/p53 pathway activation. The transcription factor reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying P53 activity in difficult-to-transfect cells including primary and/or thawed cells.
Our PhD-level staff is ready to assist you. Email us to discuss your project or obtain help selecting the right product for your research.
Email: info@lipexogen.com
Available Options:
Specifications
Key Advantages:
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system and have been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Product Data:
Figure 1 (thumbnail). HEK293FT cells were co-transfected with P53-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for human P53 (P53, right). Fluorescence microscopy images were taken 24-36 hrs later to assess GFP reporter expression in the live cells.
Have questions about this product? Need a custom modification or stable cell line? Email us and we will respond the same day.
Email: info@lipexogen.com
Details:
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| LTV-0008 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| PEG precipitation and sucrose gradient centrifugation | |
| One vial | |
| 380 μl/vial | |
| 1-5×10^6 TU/vial, depending on the variant | |
| 3×10^8 VP/ml | |
| -80oC | |
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| Apoptosis/p53 signaling pathway | |
| Species | Human/mouse |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| p53 response elements | |
| GFP, RFP, or Firefly Luciferase | |
| Promoter (Driving Selection Marker) | EF1α |
| Puromycin (Puro) or Blasticidin (BSD), or a fluorescent protein for select products | |
| Monitor p53 transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. |
*Based on infectivity on HEK293FT cells transduction units (TU).
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
| TP53 | |
| tumor protein p53 | |
| n | NM_000546 |
| Homo sapiens/mus musculus | |
| Alias | Tumor Protein P53; Cellular Tumor Antigen P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; P53 Tumor Suppressor; Tumor Suppressor P53; Li-Fraumeni Syndrome; Tumor Supressor P53; Tumor Protein 53; BMFS5; TRP53; BCC7; LFS1 |
| Annotation Page | https://www.ncbi.nlm.nih.gov/gene/?term=NM_000546 |
| Gene IDs | HGNC:HGNC:11998 Ensembl:ENSG00000141510 MIM:191170 |
| Entrez Gene Summary | “This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]“ |