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SREBP1 Reporter Lentivirus
$595.00
Fluorescent SREBP1 Reporter Lentivirus (Lipogenesis Pathway): High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent readout for human/mouse Sterol regulatory element binding transcription factor 1 (SREBP1) activity in transduced cells. The reporter construct is preferentially activated by SREBP1 and may be useful for discriminating SREBP1 and SREBP2 transcriptional activities and for studying lipogenesis pathway activation. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying SREBP1 activity in difficult-to-transfect cells including primary and/or thawed cells.
Our PhD-level staff is ready to assist you. Email us to discuss your project or obtain help selecting the right product for your research.
Email: info@lipexogen.com
Available Options:
Specifications
Key Advantages:
- High Sensitivity – LipExoGen SREBP1 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s sterol regulatory elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Product Data:
Figure 1 (thumbnail). HEK293FT cells were co-transfected with SREBP1-TAG-Puro (left panels) or SREBP1-TAR-BSD (right panels) plasmids in combination with plasmid for constitutively active SREBP1 (caSREBP1, bottom panels) or control (vector, top panels) for 24-36 hours. Fluorescence microscopy images were then taken to asses fluorescent reporter expression in the live cells.
Figure 2. Monitoring SREBP1 activity in vivo with luciferase HCT116 cells were transduced with SREBP1-TAL-Puro (LTV-0005-3S) and puromycin selected to establish a stable cell line. The luciferase reporter activity can be detected in vivo using common imaging software. The example depicts how the product could be used as a tool to validate lead compounds or screen novel SREBP1 inhibitors, or to study lipogenesis/SREBP1 pathway in vivo.
Have questions about this product? Need a custom modification or stable cell line? Email us and we will respond the same day.
Email: info@lipexogen.com
Details:
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| LTV-0005 | |
| High-titer Lentiviral Particles, third generation, VSV-G pseudotyped | |
| Serum-free RPMI-1640, frozen solution | |
| PEG precipitation and sucrose gradient centrifugation | |
| One vial | |
| 380 μl/vial | |
| 1-5×10^6 TU/vial, depending on the variant | |
| 3×10^8 VP/ml | |
| -80oC | |
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| SREBF1 (SREBP1) signaling pathway/Lipogenesis pathway | |
| Species | Human/mouse |
| Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer | |
| SREBF1 response elements (sterol regulatory elements, preference for SREBP1 over SREBP2) | |
| GFP, RFP, or Firefly Luciferase | |
| Promoter (Driving Selection Marker) | EF1α |
| Puromycin (Puro) or Blasticidin (BSD), or a fluorescent protein for select products | |
| Monitor SREBP1 transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. |
*Based on infectivity on HEK293FT cells transduction units (TU).
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
| SREBF1 | |
| sterol regulatory element binding transcription factor 1 | |
| n | NM_001005291 |
| Homo sapiens/mus musculus | |
| Alias | Sterol Regulatory Element Binding Transcription Factor 1; Sterol Regulatory Element-Binding Protein 1; Class D Basic Helix-Loop-Helix Protein 1; SREBP1; BHLHd1; Sterol Regulatory Element-Binding Transcription Factor 1; SREBP-1; BHLHD1 |
| Annotation Page | https://www.ncbi.nlm.nih.gov/gene/?term=NM_001005291 |
| Gene IDs | HGNC:HGNC:11289 Ensembl:ENSG00000072310 MIM:184756 |
| Entrez Gene Summary | “This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]“ |