Fluorescent SREBP1 Reporter Lentivirus (SREBP1-TAG-Puro and SREBP1-TAR-Puro))

SREBP1 Reporter Lentivirus

SKU: LTV-0005

$595.00

Fluorescent SREBP1 Reporter Lentivirus (Lipogenesis Pathway): High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent readout for human/mouse Sterol regulatory element binding transcription factor 1 (SREBP1) activity in transduced cells. The reporter construct is preferentially activated by SREBP1 and may be useful for discriminating SREBP1 and SREBP2 transcriptional activities and for studying lipogenesis pathway activation. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying SREBP1 activity in difficult-to-transfect cells including primary and/or thawed cells.

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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.

 

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Specifications

Key Advantages:

  • High Sensitivity – LipExoGen SREBP1 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s sterol regulatory elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
  • Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
  • Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
  • Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
  • Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.
  • Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Product Data:

Figure 1 (thumbnail). HEK293FT cells were co-transfected with SREBP1-TAG-Puro (left panels) or SREBP1-TAR-BSD (right panels) plasmids in combination with plasmid for constitutively active SREBP1 (caSREBP1, bottom panels) or control (vector, top panels) for 24-36 hours. Fluorescence microscopy images were then taken to asses fluorescent reporter expression in the live cells.

 

Figure 2. Monitoring SREBP1 activity in vivo with luciferase   HCT116 cells were transduced with SREBP1-TAL-Puro (LTV-0005-3S) and puromycin selected to establish a stable cell line. The luciferase reporter activity can be detected in vivo using common imaging software. The example depicts how the product could be used as a tool to validate lead compounds or screen novel SREBP1 inhibitors, or to study lipogenesis/SREBP1 pathway in vivo.

 

 

 

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Details:

LTV-0005
SREBF1
sterol regulatory element binding transcription factor 1
NM_001005291
Human/mouse

 

Recommended Controls

Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.

Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.

 

Custom Orders

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

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Additional Information

Additional Information

SREBF1
sterol regulatory element binding transcription factor 1
n NM_001005291
Homo sapiens/mus musculus
Alias Sterol Regulatory Element Binding Transcription Factor 1; Sterol Regulatory Element-Binding Protein 1; Class D Basic Helix-Loop-Helix Protein 1; SREBP1; BHLHd1; Sterol Regulatory Element-Binding Transcription Factor 1; SREBP-1; BHLHD1
Annotation Page https://www.ncbi.nlm.nih.gov/gene/?term=NM_001005291
Gene IDs HGNC:HGNC:11289 Ensembl:ENSG00000072310 MIM:184756
Entrez Gene Summary “This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]“