FAQs
Account
  • Log in
  • Register
0
LipExoGen
  • Home
  • Products
    • Liposome Products
      • Cationic Liposomes
      • Drug Loaded Liposomes
      • Fluorescent Liposomes
      • Immunogenic Liposomes
      • Liposomes for Click Chemistry
      • Plain (Non-PEGylated)
      • Plain (PEGylated)
      • View All
    • Gene Products
      • Lentiviral Particles
        • shRNA Lentiviral Particles
        • miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Gene Promoter Lentiviral Particles
        • Luc-Fluorescent Imaging Lentiviral Particles
        • View All
      • Plasmids
        • Non-Lentiviral ORF cDNA Expression Plasmids
        • Lentiviral ORF cDNA Expression Plasmids
        • View All
  • Services
    • Recombinant Antibody Services
    • Lipid Nanoparticles (LNPs)
    • Liposome Synthesis
      • Liposomal Formulation Development
      • Build Your Own Regular Liposomes
      • Modify A Regular Liposome Product
    • Peptide Synthesis
    • Exosome Services
      • Exosome Isolation
      • Exosome Engineering
    • Gene Services
      • Custom Lentiviral Particles
        • Validated shRNA Lentiviral Particles
        • Custom miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • LentiCRISPR sgRNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Promoter Lentiviral Particles
      • Custom Gene Cloning
        • Verified shRNA Custom Cloning
        • miRNA Custom Cloning
        • ORF cDNA Custom Cloning
        • TF Reporter Custom Cloning
        • Gene Promoter Custom Cloning
        • AAV Gene Cloning
      • Gene Mutagenesis
      • Gene Regulation
  • Support
    • Order Forms
      • Custom Regular Liposome
      • Formulation Development
      • Modify Liposome Product
      • Engineered Exosomes
      • Express Exosome Isolation
      • Gene Mutagenesis
      • Gene Regulation
      • Custom shRNA Lentiviral Particles
      • Custom miRNA Lentiviral Particles
      • Custom ORF cDNA Lentiviral Particles
      • Custom LentiCRISPR sgRNA Lentiviral Particles
      • Custom TF Reporter Lentiviral Particles
      • Custom Gene Promoter Lentiviral Particles
      • shRNA Custom Cloning
      • miRNA Custom Cloning
      • ORF cDNA Custom Cloning
      • Gene Promoter Custom Cloning
      • TF Reporter Custom Cloning
    • Supporting Documents
      • Pre-Made Vectors List
      • General Vectors List 1
      • General Vectors List 2
      • General Vectors List 3
      • Lentivirus Transduction Protocol
      • Lentivirus MSDS
    • Product FAQs
    • Technical Support
  • Research
    • Drug Development
    • Immunology and Immunotherapy
    • Oncology and Chemotherapy
    • Tumor Models
  • About
  • Contact
×
LipExoGen
0
All Categories
  • Home
  • Products
    • Liposome Products
      • Cationic Liposomes
      • Drug Loaded Liposomes
      • Fluorescent Liposomes
      • Immunogenic Liposomes
      • Liposomes for Click Chemistry
      • Plain (Non-PEGylated)
      • Plain (PEGylated)
      • View All
    • Gene Products
      • Lentiviral Particles
        • shRNA Lentiviral Particles
        • miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Gene Promoter Lentiviral Particles
        • Luc-Fluorescent Imaging Lentiviral Particles
        • View All
      • Plasmids
        • Non-Lentiviral ORF cDNA Expression Plasmids
        • Lentiviral ORF cDNA Expression Plasmids
        • View All
  • Services
    • Recombinant Antibody Services
    • Lipid Nanoparticles (LNPs)
    • Liposome Synthesis
      • Liposomal Formulation Development
      • Build Your Own Regular Liposomes
      • Modify A Regular Liposome Product
    • Peptide Synthesis
    • Exosome Services
      • Exosome Isolation
      • Exosome Engineering
    • Gene Services
      • Custom Lentiviral Particles
        • Validated shRNA Lentiviral Particles
        • Custom miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • LentiCRISPR sgRNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Promoter Lentiviral Particles
      • Custom Gene Cloning
        • Verified shRNA Custom Cloning
        • miRNA Custom Cloning
        • ORF cDNA Custom Cloning
        • TF Reporter Custom Cloning
        • Gene Promoter Custom Cloning
        • AAV Gene Cloning
      • Gene Mutagenesis
      • Gene Regulation
  • Support
    • Order Forms
      • Custom Regular Liposome
      • Formulation Development
      • Modify Liposome Product
      • Engineered Exosomes
      • Express Exosome Isolation
      • Gene Mutagenesis
      • Gene Regulation
      • Custom shRNA Lentiviral Particles
      • Custom miRNA Lentiviral Particles
      • Custom ORF cDNA Lentiviral Particles
      • Custom LentiCRISPR sgRNA Lentiviral Particles
      • Custom TF Reporter Lentiviral Particles
      • Custom Gene Promoter Lentiviral Particles
      • shRNA Custom Cloning
      • miRNA Custom Cloning
      • ORF cDNA Custom Cloning
      • Gene Promoter Custom Cloning
      • TF Reporter Custom Cloning
    • Supporting Documents
      • Pre-Made Vectors List
      • General Vectors List 1
      • General Vectors List 2
      • General Vectors List 3
      • Lentivirus Transduction Protocol
      • Lentivirus MSDS
    • Product FAQs
    • Technical Support
  • Research
    • Drug Development
    • Immunology and Immunotherapy
    • Oncology and Chemotherapy
    • Tumor Models
  • About
  • Contact
My Cart (0 )

No products in the cart.

- -
All Categories
Home Gene Products Lentiviral Particles (All) STAT3 Reporter Lentivirus
  • Previous
    Fluorescent NFkB Reporter Lentivirus (NFkB-TAG-Puro)

    NF-κB Reporter Lentivirus

    $595.00

  • Next
    STAT5 Reporter Lentivirus (STAT5-GFP-Puro)

    STAT5 Reporter Lentivirus

    $595.00

STAT3 Reporter Lentivirus

SKU: LTV-0003

$595.00

Fluorescent STAT3 Reporter Lentivirus (JAK/STAT Pathway): High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent readout for human/mouse Signal transducer and activator of transcription 3 (STAT3) activity in transduced cells. The TF reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying STAT3 activity and JAK/STAT signaling pathway in difficult-to-transfect cells including primary and/or thawed cells.

Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us

Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.

 

Categories: Gene Products, Lentiviral Particles (All), TF Reporter Lentivirus Tags: JAK/STAT Pathway, signal transducer and activator of transcription 3, STAT3

Available Options:

  • Specifications
  • Additional Information

Specifications

Key Advantages:

  • High Sensitivity – LipExoGen STAT3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s STAT3 response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
  • Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
  • Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
  • Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
  • Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.
  • Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.

Product Data:

Figure 1 (thumbnail). HEK293FT cells were co-transfected with STAT3-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for constitutively active human STAT3 (STAT3ab, right). Fluorescence microscopy images were taken 24 hrs later to assess GFP reporter expression in the live cells.

 

Figure 2. Reporter activation in stable cell line (A549) A549 cells were transduced with STAT3-TAG-Puro lentivirus (LTV-0003-1S) for 48 hours. The cells were then passaged and selected by puromycin for 5 days to establish the stable STAT3 Reporter cell line. IL6-IL6Ra fusion protein (50 ng/ml, R&D Systems) was added to the cells to visualize the GFP reporter activation by fluorescence microscopy. Note that the reporter activation will vary depending on the cells. In this instance, the proportion of cells with active STAT3 transcriptional activity is doubled in response to 24 h treatment with IL6-IL6R fusion protein whereas basal STAT3 activity (~8% of cells) is observed in the absence of stimulation. To purchase STAT3ab positive control plasmids, click here: STAT3ab Expression Plasmids (Constitutively active STAT3 positive control)

 

 

 

Have questions about this product? Send us a form and we’ll reply the same day: Contact Us

 


Details:

LTV-0003
STAT3
signal transducer and activator of transcription 3
NM_139276
Human/mouse

 


Related Products

 

 


Related Products

STAT4 Reporter Lentivirus 

STAT5 Reporter Lentivirus

STAT6 Reporter Lentivirus

GAS Reporter Lentivirus (IFNγ/JAK/STAT1 Pathway)

ISRE Reporter Lentivirus (IFNα/JAK/STAT Pathway)

 

Recommended Products

STAT3ab Expression Plasmids (Constitutively active STAT3 positive control)

Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).

Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.

Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.

 


Custom Orders

If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.

Quick Links:

Custom Lentivirus Services

Premade TF/Signal Pathway Reporter Lentivirus

All Lentivirus Products

Additional Information

Additional Information

STAT3
signal transducer and activator of transcription 3
n NM_139276
Homo sapiens/mus musculus
Alias Signal Transducer And Activator Of Transcription 3; Acute-Phase Response Factor; APRF; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Annotation Page https://www.ncbi.nlm.nih.gov/gene/?term=NM_139276
Gene IDs HGNC:HGNC:11364 Ensembl:ENSG00000168610 MIM:102582
Entrez Gene Summary “The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]”

Never Miss An Update

Subscribe to our newsletter

Products

  • Liposome Products
  • Gene Products

services

  • Liposome Services
  • Exosome Services
  • Gene Services

Information

  • Contact Us
  • FAQs
  • Terms & Conditions
  • Privacy Policy
  • Terms of Sale

Account

  • My account
  • Orders
  • Cart
  • Checkout
  • Products
Lipexogen © 2023 | Site Credits

101 North Haven St, Baltimore, MD 21224

We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok

Sebbene sia stato creato per essere l'ultimo orologio sportivo di lusso, il modello di orologio elegante Omega è abbastanza elegante per una serata in città o una giornata di orologi replica lavoro.

Omega is one of the most prominent brands in Swiss watchmaking, known for its daring designs and premium title="rolex replica watches" >rolex replica watches timepieces.

Bij het kiezen van een Omega-jurk Replica Swiss Made is het een uitdaging om uit een breed scala aan opties te rolex replica kiezen.