STAT5 Reporter Lentivirus
Fluorescent STAT5 Reporter Lentivirus (JAK/STAT Pathway): High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent readout for human/mouse Signal transducer and activator of transcription 5 (STAT5) activity in transduced cells. The TF reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying STAT5 activity and JAK/STAT signaling pathway in difficult-to-transfect cells including primary and/or thawed cells.
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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
- High Sensitivity – LipExoGen STAT5 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s STAT5 response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Figure 1 (thumbnail). HEK293FT cells were co-transfected with STAT5-TAG-Puro plus either control plasmid (Vector, left) or expression plasmid for constitutively active human STAT5 (STAT5ab, right). Fluorescence microscopy images were taken 24 hrs later to assess GFP reporter expression in the live cells.
Figure 2. STAT5 Reporter Activation/Inhibition in BaF3 Cells on Flow BaF3 cells were transduced with STAT5-TAG-Puro lentiviral particles (blue and green lines) or STAT3-TAG-Puro (black dotted line) as a negative control for STAT5. Transduction was carried out over 2 days in RPMI with 5 ng/ml IL-3, followed by selection with puromycin (2 µg/ml). The drug resistant cells transduced with STAT5-TAG-Puro were then treated with 5 µM Ruxolitinib (green line) or DMSO control (blue line) for 48 h before flow cytometry.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
|STAT5 response element|
|Monitor STAT5 signaling pathway activity, screen for inhibitors or activators of STAT5. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.|
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient services for custom cloning and lentiviral particles on demand, usually for the same or comparable price as the listed item.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
|signal transducer and activator of transcription 5A|
|Homo sapiens/mus musculus|
|Alias||Signal Transducer And Activator Of Transcription 5A; STAT5; Epididymis Secretory Sperm Binding Protein; MGF|
|Gene IDs||HGNC:HGNC:11366 Ensembl:ENSG00000126561 MIM:601511|
|Entrez Gene Summary||“The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]”|