FAQs
Account
  • Log in
  • Register
0
LipExoGen
  • Home
  • Products
    • Liposome Products
      • Cationic Liposomes
      • Drug Loaded Liposomes
      • Fluorescent Liposomes
      • Immunogenic Liposomes
      • Liposomes for Click Chemistry
      • Plain (Non-PEGylated)
      • Plain (PEGylated)
      • View All
    • Reporter Lentivirus Catalog
    • TetOn System
    • Gene Products
      • Lentiviral Particles
        • shRNA Lentiviral Particles
        • miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Gene Promoter Lentiviral Particles
        • Luc-Fluorescent Imaging Lentiviral Particles
        • View All
      • Plasmids
        • Non-Lentiviral ORF cDNA Expression Plasmids
        • Lentiviral ORF cDNA Expression Plasmids
        • View All
  • Services
    • Recombinant Antibody Services
    • Lipid Nanoparticles (LNPs)
    • Liposome Synthesis
      • Liposomal Formulation Development
      • Build Your Own Regular Liposomes
      • Modify A Regular Liposome Product
    • Exosome Services
      • Exosome Isolation
      • Exosome Engineering
    • Gene Services
      • Custom Lentiviral Particles
        • Validated shRNA Lentiviral Particles
        • Custom miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • LentiCRISPR sgRNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Promoter Lentiviral Particles
      • Custom Gene Cloning
        • Verified shRNA Custom Cloning
        • miRNA Custom Cloning
        • ORF cDNA Custom Cloning
        • TF Reporter Custom Cloning
        • Gene Promoter Custom Cloning
        • AAV Gene Cloning
      • Gene Mutagenesis
      • Gene Regulation
  • Support
    • Order Forms
      • Custom Regular Liposome
      • Formulation Development
      • Modify Liposome Product
      • Engineered Exosomes
      • Express Exosome Isolation
      • Gene Mutagenesis
      • Gene Regulation
      • Custom shRNA Lentiviral Particles
      • Custom miRNA Lentiviral Particles
      • Custom ORF cDNA Lentiviral Particles
      • Custom LentiCRISPR sgRNA Lentiviral Particles
      • Custom TF Reporter Lentiviral Particles
      • Custom Gene Promoter Lentiviral Particles
      • shRNA Custom Cloning
      • miRNA Custom Cloning
      • ORF cDNA Custom Cloning
      • Gene Promoter Custom Cloning
      • TF Reporter Custom Cloning
    • Supporting Documents
      • Pre-Made Vectors List
      • General Vectors List 1
      • General Vectors List 2
      • General Vectors List 3
      • Lentivirus Transduction Protocol
      • Lentivirus MSDS
    • Product FAQs
    • Technical Support
  • Research
    • Drug Development
    • Immunology and Immunotherapy
    • Oncology and Chemotherapy
    • Tumor Models
  • About
  • Contact
×
LipExoGen
0
All Categories
  • Home
  • Products
    • Liposome Products
      • Cationic Liposomes
      • Drug Loaded Liposomes
      • Fluorescent Liposomes
      • Immunogenic Liposomes
      • Liposomes for Click Chemistry
      • Plain (Non-PEGylated)
      • Plain (PEGylated)
      • View All
    • Reporter Lentivirus Catalog
    • TetOn System
    • Gene Products
      • Lentiviral Particles
        • shRNA Lentiviral Particles
        • miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Gene Promoter Lentiviral Particles
        • Luc-Fluorescent Imaging Lentiviral Particles
        • View All
      • Plasmids
        • Non-Lentiviral ORF cDNA Expression Plasmids
        • Lentiviral ORF cDNA Expression Plasmids
        • View All
  • Services
    • Recombinant Antibody Services
    • Lipid Nanoparticles (LNPs)
    • Liposome Synthesis
      • Liposomal Formulation Development
      • Build Your Own Regular Liposomes
      • Modify A Regular Liposome Product
    • Exosome Services
      • Exosome Isolation
      • Exosome Engineering
    • Gene Services
      • Custom Lentiviral Particles
        • Validated shRNA Lentiviral Particles
        • Custom miRNA Lentiviral Particles
        • ORF cDNA Lentiviral Particles
        • LentiCRISPR sgRNA Lentiviral Particles
        • TF Reporter Lentiviral Particles
        • Promoter Lentiviral Particles
      • Custom Gene Cloning
        • Verified shRNA Custom Cloning
        • miRNA Custom Cloning
        • ORF cDNA Custom Cloning
        • TF Reporter Custom Cloning
        • Gene Promoter Custom Cloning
        • AAV Gene Cloning
      • Gene Mutagenesis
      • Gene Regulation
  • Support
    • Order Forms
      • Custom Regular Liposome
      • Formulation Development
      • Modify Liposome Product
      • Engineered Exosomes
      • Express Exosome Isolation
      • Gene Mutagenesis
      • Gene Regulation
      • Custom shRNA Lentiviral Particles
      • Custom miRNA Lentiviral Particles
      • Custom ORF cDNA Lentiviral Particles
      • Custom LentiCRISPR sgRNA Lentiviral Particles
      • Custom TF Reporter Lentiviral Particles
      • Custom Gene Promoter Lentiviral Particles
      • shRNA Custom Cloning
      • miRNA Custom Cloning
      • ORF cDNA Custom Cloning
      • Gene Promoter Custom Cloning
      • TF Reporter Custom Cloning
    • Supporting Documents
      • Pre-Made Vectors List
      • General Vectors List 1
      • General Vectors List 2
      • General Vectors List 3
      • Lentivirus Transduction Protocol
      • Lentivirus MSDS
    • Product FAQs
    • Technical Support
  • Research
    • Drug Development
    • Immunology and Immunotherapy
    • Oncology and Chemotherapy
    • Tumor Models
  • About
  • Contact
My Cart (0 )

No products in the cart.

- -
All Categories
Home Gene Products Lentiviral Particles (All) Human IDO1 shRNA Lentivirus
  • Previous

    Human BIK shRNA Lentivirus

    $595.00

  • Next

    h/m BCL-xL shRNA Lentivirus

    $595.00 – $1,195.00

Human IDO1 shRNA Lentivirus

Human IDO1 shRNA Lentivirus

SKU: LSV-0026

$595.00 – $1,195.00

Validated human IDO1 shRNA Lentivirus: High-titer shRNA lentiviral particles specific for human indoleamine 2,3-dioxygenase 1 (IDO1). The shRNA has been validated to meet or exceed 70% IDO1 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR. The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation, and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells. Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene (>70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs.

Have questions about this product? Need stable cell line instead? Send us a form and we’ll reply the same day: Contact Us 

Categories: Lentiviral Particles (All), Validated shRNA Lentivirus

Available Options:

  • Specifications
  • Additional Information

Specifications

Key Advantages:

  • Same Cost For Custom Lentivirus – You can receive any combination of reporter (GFP/RFP/Luc/None) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of shRNA vectors, click here.
  • Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
  • Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
  • Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
  • Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.

Product Data

Construct validation for human IDO1 shRNA Lentivirus

 

 

Figure 1. Knockdown validation for hIDO1-sh2-GFP-Puro.   HEK293FT cells were co-transfected with human IDO1-RFP bicistronic expression plasmid (red) and plasmids for the indicated shRNAs (GFP, green). After 24-36 hours, fluorescent images of the living cells were acquired by fluorescence microscopy. GFP represents transfection of the shRNA construct, whereas RFP indicates translation of the target mRNA. Higher knockdown efficiencies may be possible in stably-transfected cells. Sr-sh, scrambled shRNA.

 

 

 

 

 

 

 

Figure 2. Knockdown validation for hIDO1-sh1-GFP-Puro.   HEK293FT cells were co-transfected with human IDO1-RFP bicistronic expression plasmid (red) and plasmids for the indicated shRNAs (GFP, green). After 24-36 hours, fluorescent images of the living cells were acquired by fluorescence microscopy. GFP represents transfection of the shRNA construct, whereas RFP indicates translation of the target mRNA. Higher knockdown efficiencies may be possible in stably-transfected cells. Sr-sh, scrambled shRNA. Our “set” contains sequences sh1 and sh2. Sr-sh, scrambled shRNA.

 

 

 

Have questions about this product? Send us a form and we’ll reply the same day: Contact Us

 

 


Details

LSV-0026
IDO1
indoleamine 2,3-dioxygenase 1
NM_002164.6
Human

Recommended Control

Scrambled shRNA Control Lentivirus (mixture of two independent shRNAs), LSV-0024


Custom Orders

If you require a modification to one of our products (for example, change in reporter or other vector component), please request a custom order. We provide a variety of fast and efficient services for the production of high-quality, custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable shRNA cell line for you using this product. Learn more.

The cDNA lentivirus corresponding to this product is also available upon request, comparable price.

I want to:

Go to the custom shRNA lentiviral particles request form

View all custom lentiviral particle services

Additional Information

Additional Information

IDO1
indoleamine 2,3-dioxygenase 1
n NM_002164.6
Homo sapiens
Alias IDO; INDO; IDO-1
Annotation Page https://www.ncbi.nlm.nih.gov/gene/3620
Gene IDs HGNC:HGNC:6059 Ensembl:ENSG00000131203 MIM:147435
Entrez Gene Summary “This gene encodes indoleamine 2,3-dioxygenase (IDO) – a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]”

Never Miss An Update

Subscribe to our newsletter

Products

  • Liposome Products
  • Gene Products

services

  • Liposome Services
  • Exosome Services
  • Gene Services

Information

  • Contact Us
  • FAQs
  • Terms & Conditions
  • Privacy Policy
  • Terms of Sale

Account

  • My account
  • Orders
  • Cart
  • Checkout
  • Products
Lipexogen © 2025 | Site Credits

101 North Haven St, Baltimore, MD 21224

We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok

Sebbene sia stato creato per essere l'ultimo orologio sportivo di lusso, il modello di orologio elegante Omega è abbastanza elegante per una serata in città o una giornata di orologi replica lavoro.

Omega is one of the most prominent brands in Swiss watchmaking, known for its daring designs and premium title="rolex replica watches" >rolex replica watches timepieces.

Bij het kiezen van een Omega-jurk Replica Swiss Made is het een uitdaging om uit een breed scala aan opties te rolex replica kiezen.