Transduction of Scrambled shRNA Control Lentivirus (mixed of 2 sequences).

Scrambled shRNA Control Lentivirus

SKU: LSV-0024

$395.00 – $595.00

Scrambled shRNA Control Lentivirus: High-titer shRNA lentiviral particles generated from a mixture of two independent scrambled shRNA control constructs. The scrambled shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation, and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells. Recommended for use as a control with any of our shRNA or miRNA lentiviral particles.

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Specifications

Key Advantages:

  • Same Cost For Custom Lentivirus – You can receive any combination of reporter (GFP/RFP/Luc/None) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of shRNA vectors, click here.
  • Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
  • Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
  • Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
  • Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.

Product Data

Figure 1 (thumbnail). Transduction efficiency for Scrambled shRNA control lentiviral particles   HEK293FT cells (2×10^5) in a 24-well plate were transduced with 20 µl of Scr-sh-GFP-Puro lentiviral particles for 48-72 hrs followed by image acquisition by fluorescence microscopy.

 

 

 

Figure 2. Transduction efficiency and stable cell line in floating cells. Jurkat cells (2×10^6) were transduced with 20 µl of Scr-sh-GFP-Puro lentiviral particles and GFP was detected on flow 3 days later to determine transduction efficiency (left). Dotted line represents uninfected Jurkat cells. The cells were selected using puromycin (1 µg/ml) for 3 days and then measured again on flow (right). Transduced cells can also be isolated using FACS.

 

 

 

 

 

 

 

Details

LSV-0024
N/A
N/A
N/A
Human/mouse

 

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Custom Orders

If you require a modification to one of our products (for example, change in reporter or other vector component), please request a custom order. We provide a variety of fast and efficient services for the production of high-quality, custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable shRNA cell line for you using this product. Learn more.

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