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Custom Lentiviral Particles
We offer a variety of high-quality, custom lentiviral particle services on demand. Options include lentivirus packaged with custom shRNA, miRNA, sgRNA, or ORF cDNA constructs, or with custom reporter constructs for transcription factor (TF) response elements or gene promoters. All custom lentivirus services are prompt, accurate and cost-effective. The lentiviral particles are ultra-purified to ensure the highest quality suitable for in vitro or in vivo applications. We also offer custom AAV and retroviral particles. Browse the service guide and various vector options below. To place an order or request more information, simply contact us.
Advantages of LipExoGen Custom Lentivirus Services
- Wide variety of lentiviral vectors to choose from. Click here to see our general vectors list.
- Easily establish stable cell lines expressing shRNA against your gene of interest
- Validated shRNAs – we confirm the shRNA can provide at least 70% knockdown of your gene of interest using our superior fluorescence-based assay
- Ultra-purified – all custom lentiviral particles are purified by PEG precipitation and sucrose-gradient centrifugation
- Detect transduced cells in vivo with luciferase
- Easily study gene regulation with our innovative bright fluorescent (GFP, RFP) reporter constructs
- Fast turnaround – usually 10-15 business days
- Best value – take advantage of our optimized vectors and high-quality service for similar or reduced prices vs our competitors
Vector Options
Premade Vectors
Fastest turnaround. Select the name of the destination vector you’re interested in on your form submission. View Premade Vectors List
General Vectors
We have a variety of general vectors that can be customized based on your needs. To request cloning or viral particle services using a customized version of one of our general vectors, please contact us.
Service Guide
Validated shRNA Lentiviral Particles
Custom miRNA Lentivirus
Custom cDNA ORF Lentivirus
Custom LentiCRISPR sgRNA Lentivirus
Custom Transcription Factor (TF) Reporter Lentivirus
Custom Gene Promoter Lentivirus
Validated shRNA Lentiviral Particles
Description:
High titer, purified custom lentivirus for shRNA knockdown in mammalian systems
– Easily establish stable cell lines expressing shRNA against your gene of interest
– We guarantee the clone you receive can provide at least 70% knockdown efficiency against your gene of interest
– Amount supplied sufficient for 10-20 transduction reactions*
– Receive lentivirus produced from the best shRNA clone we find to knockdown your gene from our validation procedure. Or, receive the same amount of lentiviral particles produced from a mixture of 2 validated shRNA constructs against your gene@
Tell Us:
– Gene Accession Number of the gene you want to knock down
– Your choice of destination vector
– Your choice of single validated construct or 2 pooled validated constructs
You Receive:
– High titer (2×10^7 TU/ml, 250 μl for GFP/RFP, 5×10^6 TU/ml, 250 μl for luciferase##) lentivirus producing validated shRNA against your gene of interest OR same amount of virus made by mixture of 2-3 validated constructs
– Fluorescence microscopy images confirming successful knockdown
– Sequence confirming the cloned fragment
Starting Price:
$595
Starting Turnaround:
10 business days
Custom miRNA Lentivirus
Description:
High titer, purified custom lentivirus for mammalian expression of the stem-loop or mature miRNA that you specify
– Easily establish stable cell lines expressing miRNA and recruiting miRNA to exosomes
– We guarantee 100% accuracy in our miRNA cloning
– Amount supplied sufficient for 10-20 transduction reactions*
Tell Us:
– Gene accession number of the miRNA
– Your choice of destination vector
You Receive:
– High titer (2×10^7 TU/ml, 250 μl##) custom lentivirus containing miRNA that you specify
– Sequence confirming the cloned fragment
Starting Price:
$595
Starting Turnaround:
10 business days
Custom cDNA ORF Lentivirus
Description:
Custom purified lentiviral particles containing mammalian expression construct for your desired ORF cDNA
– Amount supplied sufficient for 10-20 transduction reactions*
Tell Us:
– Gene Accession Number of the ORF cDNA fragment
– Your choice of destination vector
You Receive:
Custom lentivirus containing the ORF cDNA that you specify (2-10×10^6 TU/ml, 250-500 μl##)
– Sequence confirming the cloned fragment
– Fluorescence staining images demonstrating the cDNA expression
Starting Price:
$595
Starting Turnaround:
10 business days
Custom LentiCRISPR sgRNA Lentivirus
Description:
Fast and easy service which allows you to obtain high titer lentiviral particles for CRISPR/Cas9 knockout of your gene of interest
– We guarantee the clone you receive is functional against your gene of interest
– Amount supplied sufficient for 5-10 transduction reactions*
Tell Us:
– Choose a destination vector
– Gene Accession Number of the gene target
You Receive:
Custom purified lentivirus containing sgRNA for CRISPR/Cas9 knockout of the gene that you specify (1×10^9 lentiviral particles/ml, 2×10^6 TU/ml, 500 μl##)
– Sequence confirming the cloned fragment
Starting Price:
$695
Starting Turnaround:
10 business days
Custom Transcription Factor (TF) Reporter Lentivirus
Description:
High titer, purified lentiviral particles containing fluorescent or bioluminescent reporter construct responsive to your transcription factor of interest
– We guarantee the reporter construct is responsive to your transcription factor of interest
– Amount supplied sufficient for 10-20 transduction reactions*
Tell Us:
– Gene Accession Number for the TF
– Your choice of destination vector
– Sequence of the TF response elements known, or the short DNA range or DNA elements that the TF binds to
You Receive:
High titer (2×10^7 TU/ml***, 250 μl)## custom lentivirus containing fluorescent or bioluminescent reporter construct for your transcription factor
– Fluorescence microscopy images demonstrating activity of the reporter in response to the TF you specified
– Sequence confirming the cloned fragment
Starting Price:
$695
Starting Turnaround:
10 business days
Custom Gene Promoter Lentivirus
Description:
Custom purified lentiviral particles containing fluorescent or bioluminescent gene promoter reporter construct
– If desired, you may add enhancer/repressor sequences to the proximal promoter
– Amount supplied sufficient for 10-20 transduction reactions*
Tell Us:
– Gene Accession Number for the gene controlled by the promoter you wish to clone
– Promoter DNA fragment sequence
– Details if adding enhancer/repressor sequences of a TF
– Your choice of destination vector
You Receive:
Custom lentivirus gene promoter construct for the gene that you specify (2-10×10^6 TU/ml, 250-500 μl##)
– Sequence confirming the cloned fragment
Starting Price:
$695#
Starting Turnaround:
10 business days#
*Based on 24-well format, 1-3×10^5 cells/well reaction size and 1:1 transfection ratio. @At least one construct in the mixture guaranteed to provide 70% or more knockdown efficiency as a standalone product. Additional constructs in the mixture are not validated, but sequence-predicted based on the same method used to generate validated constructs. Knockdown validation data not provided for shRNA mixtures. ***Typical titer shown. Actual titers may vary depending on the size of the insert. #Starting price and turnaround shown for cloned DNA fragments less than 1.0 kb, without added sequences (such as enhancer or repressor sequences) and not having difficult secondary structure. ##Volumes and titers listed are estimates based on typical amounts. Actual amounts may vary.
Please note: Custom lentiviral particles are suitable for transduction of human or mouse cells. All products and services are for preclinical research use only and not intended for any therapeutic or diagnostic use. For more information, please see our Terms and Conditions.
Validated shRNA Lentiviral Particles
Starting price: $595
We provide high-titer custom lentiviral particles producing shRNAs for knockdown of your gene of interest in mammalian systems. We guarantee the clone you receive will have at least 70% knockdown efficiency because we validate the clones using a specific fluorescence-based reporter assay that is more reliable than standard qPCR. You don’t have to know any shRNA sequences at all to get started – simply tell us the gene accession number of the gene you want to knock down, and select one of our high-quality, new-version lentiviral vectors.
Our lentiviral particles can allow for the easy establishment of stable cell lines expressing shRNA. If desired, we can provide lentiviral particles engineered to recruit shRNA to exosomes.
After your order is confirmed, you can expect your custom lentivirus to be shipped out in about 10 business days. Knockdown validation data are also included as determined by our unique fluorescence microscopy assay.
To view our catalog of pre-made validated shRNA lentivirus, click here.
Custom miRNA Lentivirus
Starting price: $595
We provide high-titer custom lentiviral particles for expression of the stem-loop or mature miRNA of your choice. We guarantee the clone you receive is functional for the expression of the mature miRNA you specified. Our lentiviral particles can also allow you to easily establish stable cell lines expressing miRNA and recruiting miRNA to exosomes.
Simply tell us the gene accession number of the miRNA, and select one of our high-quality, new-version lentiviral vectors. After your order is confirmed, you can expect your custom lentivirus to be shipped out in about 10 business days.
Custom cDNA ORF Lentivirus
Starting price: $595
We provide high-titer custom lentiviral particles for expression of your specified ORF cDNA fragment. We guarantee the clone you receive is functional for the expression of the cDNA you specified.
We have created lentiviral vectors that express large size ORFs (3-6 kb) and generate much higher viral titers than currently used lentiviral vectors.
If you can send us the cDNA plasmid, the price will be comparable to our regular listed ORF cDNA lentiviruses.
Simply tell us the gene accession number of the ORF cDNA fragment, and select one of our high-quality, new-version lentiviral vectors. After your order is confirmed, you can expect your custom lentivirus to be shipped out in about 10 business days.
Custom LentiCRISPR sgRNA Lentivirus
Starting price: $695
This is a fast and easy service which allows you to obtain high titer lentiviral particles customizable for CRISPR/Cas9 knockout of your gene of interest.
We’ll make two sgRNA sequences flanking the target site and clone them each to our all in one Cas9 sgRNA vector. Then, we’ll package the lentiviral particles using a mixture of the two vectors, in order to enhance the knockout efficiency. The titer of lentivirus is about 1×10^9 lentiviral particles in 500 μl, and a total TU of about 1×10^6.
To begin, just enter the gene accession number for the gene you want to knock-out.
After your order is confirmed, you can expect your custom lentivirus to be shipped out in about 10 business days.
Custom TF Reporter Lentivirus
Starting price: $595
Take your gene regulation research to a new level with custom high titer lentiviral particles for mammalian expression of a fluorescent/bioluminescent reporter system responsive to your transcription factor (TF) of interest. We guarantee a functional reporter construct that is responsive to your TF of interest. Simply tell us the gene accession number for the TF, select one of our high-quality new-version lentiviral vectors and, if known, provide sequences of the TF response elements or the short DNA range or DNA elements the TF binds to.
After your order is confirmed, you can expect your custom lentivirus to be shipped out in about 10 business days. As a part of our quality control process, we’ll also provide fluorescence microscopy images demonstrating the reporter activity.
To view our catalog of pre-made lentiviral reporters for TFs/signal pathways, click here.
Custom Gene Promoter Lentivirus
Starting price: $595*
Take your gene regulation research to a new level with custom lentiviral particles for mammalian expression of a fluorescent/bioluminescent reporter system driven by the human or mouse proximal promoter of your choice. This service also lets you add enhancer/repressor sequences to the proximal promoter if desired.
Simply tell us the gene accession number for the gene controlled by the promoter you wish to clone, the sequence of the promoter DNA fragment, any details if adding additional regulatory elements, and select one of our high-quality new-version lentiviral vectors. After placing your order, you can expect to receive your custom lentiviral particles in as fast as 10 business days.
Other Services
Design your own exosomes engineered with peptide-based surface molecules, or send us your conditioned medium for express exosome isolation.
Whether you’re seeking regular plain or PEGylated liposomes, or complex formulations with drugs, dyes, and more, our team of liposome experts is ready to tackle your project with the dedication and accuracy you deserve.