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Gene Cloning Services
Our Gene Cloning services are ideal for anyone seeking mammalian expression plasmids. We are committed to providing you the highest quality at an affordable price and with fast turnaround times. We offer a wide variety of new-version regular, lentiviral, or AAV vectors that ensure you will receive the absolute best quality products available today. All you need to do is provide the gene accession number and choose one of our unique destination vectors – then we will prepare and ship you the sequence-verified plasmid DNA containing your insert.
Vector Options:
Premade vectors (fastest turnaround). Simply indicate the name of the vector you’re interested in on your form submission. View Premade Vectors List
General Vectors
We have a variety of general vectors that can be customized based on your needs. To request custom cloning using one of our general vectors, contact us.
ADVANTAGES OF LIPEXOGEN GENE CLONING SERVICES
- Easily establish stable cell lines
- Easily produce high-titer lentivirus capable of transducing primary and other difficult-to-transfect cells
- Easy Ordering – just tell us what you want to do, and we can usually design the most efficient shRNAs, reporter systems, etc. for you which lets you save time
- Affordable Pricing - Prices are comparable to purchasing commercially-available expression plasmids
- All of our vectors have been optimized for maximum expression in transient transfection
- All of our lentiviral vectors have been optimized to yield high virus titers and gene expression, making it easy to establish long-term, stable cell lines
- More Flexibility – Regular vectors are premade for easy customizability for tag location (N-terminal or C-terminal to the insert)
- All custom shRNAs are verified by our own fluorescence-based system that is more reliable than RT-qPCR – as a result, we guarantee at least 70% knockdown efficiency
- All cloning is sequence chromatogram confirmed, ensuring 100% accuracy
- We match competitor’s prices – if you find the same quality of service for cheaper, tell us
- We provide turnaround times that are comparable to or faster than the competition
*Starting price shown for regular vectors and insert less than 1 kb; starting price for lentiviral vectors is $795. Actual prices and turnaround will depend on several factors, including cloned fragment length and complexity, type of destination vector requested, etc. If requesting a cloned fragment >4 kb and you already have a cDNA clone, you may optionally send one to us which may reduce your turnaround time and cost. If the gene you want us to clone is only expressed in special cells or under specific conditions, (i.e. the cDNA cannot easily be obtained by general RT-PCR), you should notify us of this when placing your request. In such cases we may require that you provide freshly isolated high-quality RNA stored as an RNA pellet in isopropanol.
For more details or to ask us any specific questions about your order, please contact us.
Gene Cloning Service Guide
Verified shRNA Custom Cloning
miRNA Custom Cloning
ORF cDNA Custom Cloning
Transcription Factor (TF) Reporter Custom Cloning
Gene Promoter Custom Cloning
Verified shRNA Custom Cloning
Description:
– shRNA cloning service, in which we design and clone an efficient shRNA sequence into one of our high-quality lentiviral or AAV vectors and verify its successful activity to knock down your gene of interest. – Supplied with quality control data confirming successful knockdown of the target gene by the construct we madeTell Us:
– Gene accession number of the gene you want to knock down – Your choice of destination vectorYou Receive:
– 2 µg plasmid DNA encoding shRNA against your gene of interest – Fluorescence microscopy images confirming successful knockdown* – Sequence confirming the cloned fragmentStarting Price:
$795Starting Turnaround:
10 business days MORE DETAILS ORDER NOWmiRNA Custom Cloning
Description:
Custom cloning of the stem-loop or mature miRNA sequence of your choice into one of our high-quality lentiviral or AAV vectors. – We guarantee 100% accuracy in our miRNA cloningTell Us:
– Gene accession number of the miRNA – Your choice of destination vectorYou Receive:
– 2 µg plasmid DNA encoding the stem-loop or mature miRNA of interest – Sequence confirming the cloned fragmentStarting Price:
$795Starting Turnaround:
7 business days MORE DETAILS ORDER NOWORF cDNA Custom Cloning
Description:
Custom cloning of your specified ORF cDNA fragment sequence into one of our regular, lentiviral or AAV expression vectors.Tell Us:
– Gene accession number of the ORF cDNA fragment – Your choice of destination vector (regular and lentiviral vectors available)You Receive:
– 2 µg plasmid DNA encoding your specified ORF cDNA of interest – Sequence confirming the cloned fragment – Fluorescence staining images demonstrating successful protein expressionStarting Price:
$495*Starting Turnaround:
15 business days MORE DETAILS ORDER NOWTranscription Factor (TF) Reporter Custom Cloning
Description:
Custom cloning service in which we design a lentiviral fluorescent or bioluminescent (luciferase) reporter system that is responsive to your TF of interest. – Supplied with quality control data demonstrating successful activity of the reporter in response to the TF you specifiedTell Us:
– Gene accession number for the TF – Your choice of destination vector – Sequence of the TF response elements if known, or the short DNA range or DNA elements that the TF binds toYou Receive:
– 2 µg lentiviral DNA construct encoding fluorescent or bioluminescent (luciferase) reporter system for your transcription factor of interest – Fluorescence microscopy images demonstrating successful activity of the reporter in response to the TF you specified – Sequence confirming the cloned fragmentStarting Price:
$795Starting Turnaround:
10 business days MORE DETAILS ORDER NOWGene Promoter Custom Cloning
Description:
Custom cloning service in which we clone a promoter fragment sequence specified by you into one of our lentiviral expression vectors. – If desired, you can add enhancer/repressor sequences to the proximal promoter – We guarantee the construct contains the specified promoter sequence plus any additional regulatory elements with 100% accuracyTell Us:
– Gene accession number for the gene controlled by the promoter you wish to clone – Promoter DNA fragment sequence – Details if adding enhancer/repressor sequences of a TF – Your choice of destination vectorYou Receive:
– 2 µg lentiviral DNA construct encoding fluorescent reporter system driven by your gene promoter sequence of interest and any additional regulatory elements you specify – Sequence confirming the cloned fragment – Fluorescence images demonstrating the promoter activityStarting Price:
$795Starting Turnaround:
10 business days* MORE DETAILS ORDER NOWStarting price shown for regular vectors and insert less than 1 kb; starting price for lentiviral vectors is $795. Actual prices and turnaround will depend on several factors, including cloned fragment length and complexity, type of destination vector requested, etc. If requesting a cloned fragment >4 kb and you already have a cDNA clone, you may optionally send one to us which may reduce your turnaround time and cost. If the gene you want us to clone is only expressed in special cells or under specific conditions, (i.e. the cDNA cannot easily be obtained by general RT-PCR), you should notify us of this when placing your request. In such cases we may require that you provide freshly isolated high-quality RNA stored as an RNA pellet in isopropanol.
*Fluorescence microscopy validation is not possible for requests for shRNA cloning targeting non-coding regions. Instead, we will provide two clones instead of one, for no additional cost. If you wish to order custom shRNA cloning targeting a non-coding region, please note this in the order form.
For more details or to ask us any specific questions about your order, please contact us.
Verified shRNA Custom Cloning
Starting price: $795
With our Verified shRNA Custom Cloning service, we will design and clone an efficient shRNA sequence into one of our high-quality lentiviral or AAV vectors and verify its successful activity to knockdown your gene of interest. The construct is verified by transfection and a fluorescence-based assay and the quality control data confirming knockdown is provided along with sequence confirming the cloned fragment.
To request this service, please submit a form using the button above and provide us the gene accession number for the gene you want to knock down, along with your choice of one of our new-version vectors. Starting turnaround for this service is 10 business days.
miRNA Custom Cloning
Starting price: $795
With our miRNA Custom Cloning service, we will clone the stem-loop or mature miRNA sequence of your choice into one of our high-quality lentiviral or AAV vectors. We guarantee the clone you receive is functional for the expression of the mature miRNA you specified.
To request this service, please submit a form using the button above and provide us the gene accession number of the miRNA along with your choice of one of our new-version vectors. Starting turnaround is 7 business days.
ORF cDNA Custom Cloning
Starting price: $495
With our ORF cDNA Custom Cloning service, we will clone your specified ORF cDNA fragment sequence into one of our regular, lentiviral, or AAV vectors, guaranteed to functionally express the cDNA fragment you specified.
To request this service, please submit a form using the button above and provide us the gene accession number of the ORF cDNA fragment and choose one of our new-version vectors. Starting turnaround is 15 business days. Price refers to non-lentiviral vectors.
Recombinant Antibodies
Starting price: $4995
We provide custom recombinant antibodies for preclinical use in vitro or in vivo. Simply provide us the heavy and light chain variable region sequences and let us take care of the rest. We’ll clone the sequence to our own high-efficiency vectors which allow us to provide mg quantities of custom antibodies for a competitive price.
To request this service, please contact us.
Gene Promoter Custom Cloning
Starting price: $795
With our Gene Promoter Custom Cloning service, we will clone a promoter fragment sequence that you specify into one of our lentiviral vectors. This service also lets you add regulatory elements such as enhancer or repressor sequences to the proximal promoter as desired. We guarantee 100% accuracy in our cloning.
To request this service, please submit a form using the button above and provide us the gene accession number for the gene under control of the promoter of interest and the promoter DNA fragment sequence. If you want to further customize the construct with additional regulatory elements, please provide details. Finally, choose one of our new-version vectors. Starting turnaround is 10 business days.
Lentivirus Products
Designed to let you boldly enter new fields, our services spark innovation and drive your discoveries towards commercialization.
Other Gene Services
Our Gene Mutagenesis service is a convenient way to get mutant DNA clones by site-directed mutagenesis while avoiding tedious kits and benchwork.
Our comprehensive Gene Regulation Services are an ideal solution for researchers who are interested in studying gene regulation by transcription factors.