Reporter Negative Control Lentivirus

Reporter Negative Control Lentivirus: The reporter negative control is useful for determining background reporter activity when it is used with one of the regular TF Reporter lentivirus products. For TF reporters that show strong basal activity (i.e. without any stimulation), the negative control can be used to confirm the activity is specific to the presence of response elements in the construct. For TF reporters showing weak activation under your particular conditions, the negative control can help confirm that the reporter signal is pathway specific weak activation and not an off-target effect. The negative control construct contains all the components of the regular TF reporters except for the response elements that otherwise drive signal pathway specific reporter expression. An EF1a-driven selection marker is included like all of the regular TF reporters to allow you to easily establish stable cell lines. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for use with difficult-to-transfect cells including primary and/or thawed cells.

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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.