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Reporter Negative Control Lentivirus
$595.00
Reporter Negative Control Lentivirus: The reporter negative control is useful for determining background reporter activity when it is used with one of the regular TF Reporter lentivirus products. For TF reporters that show strong basal activity (i.e. without any stimulation), the negative control can be used to confirm the activity is specific to the presence of response elements in the construct. For TF reporters showing weak activation under your particular conditions, the negative control can help confirm that the reporter signal is pathway specific weak activation and not an off-target effect. The negative control construct contains all the components of the regular TF reporters except for the response elements that otherwise drive signal pathway specific reporter expression. An EF1a-driven selection marker is included like all of the regular TF reporters to allow you to easily establish stable cell lines. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for use with difficult-to-transfect cells including primary and/or thawed cells.
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Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
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Specifications
Details
| LTV-0071 | |
| N/A | |
| N/A (no response elements) | |
| The reporter negative control lentivirus is useful for determining background reporter activity when it is used with one of the TF Reporter lentivirus products. For TF reporters that show strong basal activity (i.e. without any stimulation), the negative control can be used to confirm the activity is specific to the presence of response elements in the construct. For TF reporters that show weak activation under your particular conditions, the negative control can help to confirm that the reporter signal is pathway specific weak activation and not an off-target effect. The negative control contains all the components of the regular TF reporters construct except for the response elements that otherwise drive signal pathway specific reporter expression. | |
| Human/mouse |
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Related Products
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand, usually for the same or comparable price as the listed item.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
ORF cDNA plasmids featured in the product figures are available upon request.
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Additional Information
Additional Information
- High Sensitivity – LipExoGen FOXO3 Reporter lentiviral particles are made using a novel vector platform based on the third generation system. Transcriptional response elements which are preferentially recognized by FOXO3 are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the activity of the indicated transcription factor or signaling pathway.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Vector Diagram
Vector Diagram
Size: 6.5-8.0 kb