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PU.1 Reporter Lentivirus
$595.00
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EGR1 Reporter Lentivirus
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RUNX1 Reporter Lentivirus
$595.00
RUNX1 Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse RUNX family transcription factor 1 (RUNX1) transcriptional activity in mammalian cells. The RUNX1 reporter is constructed with tandem repeats of consensus RUNX1 DNA binding elements to read out its transcriptional activity. As a member of the core-binding factor family of transcription factors, RUNX1, also known as acute myeloid leukemia 1 protein (AML1), modulates cell proliferation, differentiation, and survival. RUNX1 plays an indispensable role in definitive hematopoiesis and hematological malignancies and as an emerging therapeutic target in cardiovascular disease. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying RUNX1 transcriptional activity in difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Details
| LTV-0085 | |
| RUNX1 Signaling Pathway | |
| RUNX1 response element | |
| Monitor transcriptional activity of RUNX1 in mammalian cells, screen for activators or inhibitors of RUNX1 transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Product Data
Figure 1. GFP reporter activation in HEK293FT cells co-transfected with RUNX1 -TAG-Puro reporter and human RUNX1. HEK293FT cells were transfected with RUNX1-TAG-Puro plasmid along with cDNA encoding human RUNX1 or an empty vector (pcDNA). The RUNX1 cDNA vector (RUNX1-P2A-RFP) contains RFP downstream of RUNX1 separated by a P2A sequence. Fluorescence microscopy was performed 24-36 h after transfection. This product is supplied as pre-packaged lentiviral particles with your choice of reporter and selection marker. RUNX1 cDNA expression vectors is also available upon request. For more information, please email info@lipexogen.com.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, contact us. We provide fast and efficient custom lentiviral particles on demand.
Or, send us your cells and we will establish a stable reporter cell line for you using this product. Learn more.
ORF cDNA plasmids featured in the product figures are available upon request.
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