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FXRE Reporter Lentivirus
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SRE Reporter Lentivirus
$595.00
Fluorescent Serum Response Element (SRE) Reporter Lentivirus: High-quality, fluorescent lentiviral reporter system for MAPK/ERK and growth factor signaling pathways in mammalian cells. Tandem repeats of the c-Fos SRE drive expression of the fluorescent reporter to readout transcriptional activity of serum response factor (SRF) and its cofactors (e.g. ELK-1). The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying activation of MAPK/ERK and growth factor signaling pathways in difficult-to-transfect cells including primary and/or thawed cells.
Have questions about this product? Need a stable cell line? Send us a form and we’ll reply the same day: Contact Us
Ultra-high concentration virus can be provided upon request in your choice of medium and volume for in vivo injection. We can provide custom reporter genes (such as SEAP, YFP, secreted Gaussia Luc, Renilla Luc, etc.) and selection markers (hygromycin, bleomycin, etc.) upon request.
Available Options:
Specifications
Key Advantages:
- High Sensitivity – LipExoGen TF Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized minimal enhancer (pc) of the human CMV promoter. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.
- Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.
- Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout directly by fluorescence microscopy in living cell cultures, thus paving the way for unexpected discoveries.
- Readout On Flow – Fluorescent reporter activation can also be readout by flow cytometry, providing more versatility in data acquisition for labs with different instruments.
- Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system and have been validated to read out the indicated transcription factor activity.
- Same Cost For Custom Lentivirus – You can easily request any combination of reporter (GFP/RFP/Luc) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of vectors, click here.
Product Data:
Figure 1 (thumbnail). HEK293FT cells were transfected with SRE-TAG-Puro construct for 24 hrs, then stimulated with PMA or DMSO for 12 hrs. Then, fluorescence microscopy images were taken to assess GFP reporter expression in the live cells.
Figure 2. SRE-TAG-Puro Activation by Serum HEK293FT cells were transfected with SRE-TAG-Puro construct for 24 h. After 8 h serum starvation, the cells were supplemented overnight with either 1% or 20% FBS in DMEM to show the effects of increasing serum concentrations.
Figure 3. SRE-TAL reporters enable in vivo quantitation of MAPK/ERK pathway and provide a sensitive platform to study pharmacodynamics of putative inhibitors SRE-TAL-Puro lentiviral particles were used to establish HCT116 stable cell line expressing firefly luciferase in response to MAPK/ERK pathway activity. The IVIS image in (A) shows the reporter activity 10 minutes after luciferin injection in NSG recipients. The animals in the left panels of (A) received empty liposomes as a control, whereas animals in the right side panels received liposomes loaded with the MAPK/ERK inhibitor PD0325901 and fluorescently tagged with DiD (product SKU DLL-0008-1B). Top and bottom panels show the luminescence signal intensities before and after (72 h later) administration of the liposomes. The fluorescence channel for the same animals that received DiD labeled liposomes in (A) is shown in (B), depicting the accumulation of DiD labeled liposomes in HCT116 tumor tissues. The images in (B) and the bottom panels of (A) were acquired in the same session.
Have questions about this product? Send us a form and we’ll reply the same day: Contact Us
Details:
| LTV-0028 | |
| MAPK/ERK | |
| Serum response element (SRE) | |
| Monitor MAPK/ERK pathway activity. The reporter is also activated by serum and growth factors. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. | |
| Human/mouse |
Vector Diagram
Recommended Controls
Reporter Negative Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven reporter. The construct is the same as the TF Reporters except that it lacks the transcriptional response elements which drive signal pathway/TF-specific reporter expression. The reporter negative control lentiviral particles allow to establish a baseline for background reporter activity and determine specificity of any treatments to activate the reporter.
Reporter Positive Control Lentivirus – Ready-to-transduce lentiviral particles with constitutive expression of the reporter. The reporter positive control lentivirus is useful for transduction optimization studies, especially if the cells are very sensitive or difficult-to-transduce.
Renilla Luciferase Internal Control Lentivirus – Ready-to-transduce lentiviral particles expressing minimal TATA box-driven Renilla luciferase (RLuc).
Publications
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us. Examples of customization options are shown in the table below. Feel free to request something not in the table.
Additional Custom Service Options
- Send us your cells and we can establish a stable NFAT reporter cell line for you using this product. Learn more.
- ORF cDNA plasmids featured in the product figures are available upon request.
- Ultra-high concentration NFAT reporter virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
Additional Information
Additional Information
| SRF | |
| Serum response factor | |
| n | NM_003131.4 |
| Homo sapiens/mus musculus | |
| Alias | MCM1 |
| Annotation Page | https://www.ncbi.nlm.nih.gov/gene/6722 |
| Gene IDs | HGNC:HGNC:11291 Ensembl:ENSG00000112658 MIM:600589 |
| Entrez Gene Summary | “This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]” |
Vector Map
Vector Map
Size: 6.5-8.0 kb