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hP53 sgRNA-SpCas9 Lentivirus
$695.00
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Lentiviral Promoter Reporter Kit
Price range: $95.00 through $895.00
The LipExoGen Lentiviral Promoter Reporter Kit represents the first-ever lentiviral-based promoter reporter vector, an innovative tool designed to detect gene promoter activity and regulation directly within cells that endogenously express the gene. Understanding how transcription factors regulate genes at multiple levels is crucial for advancing research on gene expression and regulation. To fulfill this process it is necessary to use lentivirus to introduce the reporter gene into the cells that endogenously express the gene. The Lentiviral Promoter Reporter Kit allows you to insert your promoter of interest into our linearized reporter vector which can then be packaged into lentiviral particles for the study.
- Reporters Included: Choose from GFP, RFP, or Luc to readout promoter activity.
- Selection Markers: Choose from Puro or BSD drug selection markers driven by the EF1a promoter.
- High-Titer Lentivirus Production: Modified to work seamlessly with our packaging system, this vector overcomes the challenges of transducing difficult cell types.
- Flexibility in Transfection: While optimized for use in lentiviral transduction, the promoter reporter can also be effectively used in transient transfection with plasmids.
You can customize your kit by choosing alternative reporters or selection markers to meet your specific research needs.
Available Options:
Specifications
Product Data
Figure 1 (Thumbnail) HEK293 FT cells were co-transfected with human FASN promoter reporter (FASN-P-GFP-Puro) plasmid along with cDNA encoding constitutively active human SREBP1a (caSREBP1a) or pcDNA3 empty vector plasmids for 32 hours before acquiring fluorescence microscopy images. Some pcDNA3-transfected cells show endogenous FASN promoter activity without exogenously expressed caSREBP1a. The FASN promoter contains the binding sites for SREBP1, a TF which is well known for its ability to induce FASN gene expression. The proximal region of human FASN promoter was cloned into a Lenti-promoter reporter vector, EF1a-Puro-Prom-GFP.
Custom Orders
If you require a modification to one of our products, such as a change in reporter or other vector component, please contact us.
Additional Custom Service Options
- Send us your cells and we can establish a stable reporter cell line for you using this product. Learn more.
- ORF cDNA featured in the product figures are available upon request, supplied as plasmid or high-titer lentivirus.
- Ultra-high concentration virus can be provided upon request in your choice of medium and volume (i.e. for in vivo applications).
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Additional Information
Additional Information
- Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
- Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
- Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
- Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.
Vector Diagram
Vector Diagram
Size: 8.5 kb
The shRNA is driven by human U6 promoter. The EF1a promoter separately drives constitutive expression of a reporter gene and drug selection marker, separated by P2A.
Our Validation Process
Our Validation Process
