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Home Gene Products Lentiviral Particles (All) h/m FASN shRNA Lentivirus
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    $595.00 – $1,195.00

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h/m FASN shRNA Lentivirus

SKU: LSV-0012

$595.00 – $1,195.00

Validated h/m FASN shRNA Lentivirus: High-titer shRNA lentiviral particles specific for human/mouse fatty acid synthase (FASN). The shRNA has been validated to meet or exceed 70% FASN knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR. The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation, and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells. Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene (>70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs.

Have questions about this product? Need stable cell line instead? Send us a form and we’ll reply the same day: Contact Us 

Categories: Lentiviral Particles (All), Featured Lentivirus, Validated shRNA Lentivirus

Available Options:

  • Specifications
  • Additional Information

Specifications

Key Advantages:

  • Same Cost For Custom Lentivirus – You can receive any combination of reporter (GFP/RFP/Luc/None) and selection marker (puromycin/blasticidin) for this product, without additional cost, by contacting us. To view our complete list of shRNA vectors, click here.
  • Superior knockdown – LipExoGen Validated shRNA Lentiviruses are produced using the third generation system and feature novel, optimized shRNA vectors which express a 19-20 bp shRNA, fluorescent (GFP or RFP) or luminescent (luciferase) reporter, and drug-selection marker (puromycin or blasticidin). Taking advantage of a proprietary prediction algorithm developed in-house, validated shRNA constructs are capable of delivering 70% or more knockdown efficiency with less off-target effects compared to longer or mixed-sequence shRNA/siRNAs.
  • Superior validation – All of our pre-made shRNA constructs are validated in-house using a specific fluorescence-based method that is more reliable than traditional qPCR. The validation process leverages bicistronic expression of the target mRNA and fluorescent reporter to confirm the efficacy of the shRNA. As knockdown validation can be readout using basic fluorescence microscopy, this low-cost, streamlined approach allows us to provide a superior-quality product at a price comparable or less than the average competitor.
  • Superior accuracy – Polyclonal shRNA-transduced stable cells can be established within 10 days and used for downstream applications while preserving more properties of the parental cells. In this way, high-efficiency knockdown from our validated shRNA lentiviral particles can be advantageous over sgRNA CRISPR-Cas9 systems which select for single cell clones.
  • Easily identify transduced cells – Validated shRNA constructs contain both fluorescent reporter and drug selection marker, allowing the flexibility to select transduced cells by puromycin/blasticidin or FACS sorting of GFP/RFP. Luciferase reporters are also available for detecting transduced cells in vitro or in vivo using luminescence-based techniques.

Product Data

Figure 1 (thumbnail). Transduction efficiency for h/m FASN shRNA lentiviral particles   HEK293FT cells (2×10^5) in a 24-well plate were transduced with 20 µl of h/m FASN-sh-RFP-BSD lentiviral particles for 48-72 hrs, followed by image acquisition by fluorescence microscopy.

 


Details

LSV-0012
FASN
fatty acid synthase
NM_004104
Human/mouse

Recommended Control

Scrambled shRNA Control Lentivirus (mixture of two independent shRNAs), LSV-0024

(Included free with your purchase of an shRNA lentivirus kit (SKU ending in “KIT”).

 


Custom Orders

If you require a modification to one of our products (for example, change in reporter or other vector component), please request a custom order. We provide a variety of fast and efficient services for the production of high-quality, custom lentiviral particles on demand, usually for the same or comparable price as the listed item.

Or, send us your cells and we will establish a stable shRNA cell line for you using this product. Learn more.

I want to:

Go to the custom shRNA lentiviral particles request form

View all custom lentiviral particle services

Additional Information

Additional Information

FASN
fatty acid synthase
n NM_004104
Homo sapiens
Alias Fatty Acid Synthase; Short Chain Dehydrogenase/Reductase Family 27X, Member 1; FAS; EC 2.3.1.85; EC 6.3.3.1; EC 2.3.1; SDR27X1; OA-519
Annotation Page https://www.ncbi.nlm.nih.gov/gene/?term=NM_004104
Gene IDs HGNC:HGNC:3594 Ensembl:ENSG00000169710 MIM:600212
Entrez Gene Summary “The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]“

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