Key Advantages:

• Ultra-Sensitive Reporters – Our catalog contains an ever-growing array of transcription factor (TF)/signal pathway reporters – all of which feature our innovative enhanced vector system. Upon pathway activation, a mini-enhancer and up to 8 tandem repeats of TF response elements synergistically amplify expression of the reporter (GFP/RFP/Luc), providing a sensitive approach for you to study gene expression or signal pathway activation in mammalian cells. Your choice of selection marker (Puro/BSD) is driven by EF1a. Browse all of our signal pathway/TF reporters in the table below.
• High-Specificity and Sensitivity – LipExoGen offers GAL4-NR-LBD assay lentiviral reporters designed to accurately track transcriptional activity of nuclear receptors, making them ideal for agonist/antagonist drug screening. Additionally, our Tango Assay Reporter Platform provides high-sensitivity monitoring of GPCR interactions with both agonists and antagonists, streamlining drug discovery.
• Superior Knockdown – We Validate All of Our shRNAs LipExoGen Validated shRNA Lentiviruses feature novel, optimized vectors expressing 19-21 bp shRNA with your choice of reporter (GFP/RFP/Luc/None) and drug-selection marker (puromycin or blasticidin). All shRNA are guaranteed to knockdown the indicated gene by at least 70%. All of our shRNA are validated in-house using our proprietary fluorescence-based method that is more faster and more reliable than qPCR. Available as single validated shRNA, or a set of 2 validated shRNAs plus 2 scrambled controls. Click here to knock down any gene!
• Versatile readout of gene expression – Our ORF cDNA constructs are New-Version Vectors that have been optimized for production of high-titer lentiviral particles and easy establishment of stable cell lines. All of our ORF cDNA constructs feature an epitope tag (e.g. Flag, V5, Myc, HA, etc.) fused to the ORF C-terminus, allowing easy purification or staining of fixed cells/lysates. In live cells, drug selection markers allow stable cell line generation in ~10 days; or, fluorescent reporter can allow isolation by FACS.  Track cells in vivo or in vitro using fluorescence or luciferase. Self-cleaving peptides are included to allow independent protein expression of these components from a single mRNA transcript.
• High-titer & ultra purified – LipExoGen  lentiviral particles are ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation. They are suitable for transduction of primary cells, stem cells, and difficult-to-transfect cells including primary or thawed cells.
• 100% Customizable – LipExoGen lentiviral particles can be customized according to your requirements for a price that is comparable to that shown on the website.

For immunotherapy/functional assay reporter lentiviruses, if the receptor cDNA ORF exceeds 1.2 kb in the all-in-one dual-reporter construct (e.g. GFP-GLuc), transduction efficiency will be largely reduced, due to it exceeds our packaging capacity. In such cases, we provide the cDNA ORF lentivirus and the reporter lentivirus as a set instead. You can use this set for co-transfection, ensuring high-efficiency transduction without additional time delays. The price for this set is $1200.

If you need a cDNA expression plasmid that isn’t listed in the table, you may request any cDNA sequence. For cDNA size under 2.5 kb, pricing will be similar to the listed options. Larger cDNAs may incur additional costs. You can also specify your preferred vector, and the price will remain comparable for plasmids with cDNAs under 2.5 kb. Self-cleaving peptide shorthand: T2A and P2A sequences are denoted using slashes (“/”) in ORF cDNA product names. Example: Gene-V5/Reporter/SelectionMarker represents Gene-V5–T2A–Reporter–P2A–SelectionMarker. We use the “-” symbol to indicate fusions. Example: “Gene-Tag” means the tag is fused to the C-terminal of the ORF cDNA, while “Tag-” means the tag is fused to the N-terminal. You can view the diagram of the construct layout here. Please note that all of our vectors use EGFP or mCherry. If it says GFP it means EGFP. If it says RFP, it means mCherry. Abbreviations: Puro, puromycin; BSD, blasticidin.