Key Advantages:

• Ultra-Sensitive Reporters – Our catalog contains an ever-growing array of transcription factor (TF)/signal pathway reporters – all of which feature our innovative enhanced vector system. Upon pathway activation, a mini-enhancer and up to 8 tandem repeats of TF response elements synergistically amplify expression of the reporter (GFP/RFP/Luc), providing a sensitive approach for you to study gene expression or signal pathway activation in mammalian cells. Your choice of selection marker (Puro/BSD) is driven by EF1a. Browse all of our signal pathway/TF reporters in the table below.
• High-Specificity and Sensitivity – LipExoGen offers GAL4-NR-LBD assay lentiviral reporters designed to accurately track transcriptional activity of nuclear receptors, making them ideal for agonist/antagonist drug screening. Additionally, our Tango Assay Reporter Platform provides high-sensitivity monitoring of GPCR interactions with both agonists and antagonists, streamlining drug discovery.
• Superior Knockdown – We Validate All of Our shRNAs LipExoGen Validated shRNA Lentiviruses feature novel, optimized vectors expressing 19-21 bp shRNA with your choice of reporter (GFP/RFP/Luc/None) and drug-selection marker (puromycin or blasticidin). All shRNA are guaranteed to knockdown the indicated gene by at least 70%. All of our shRNA are validated in-house using our proprietary fluorescence-based method that is more faster and more reliable than qPCR. Available as single validated shRNA, or a set of 2 validated shRNAs plus 2 scrambled controls. Click here to knock down any gene!
• Versatile readout of gene expression – Our ORF cDNA constructs are New-Version Vectors that have been optimized for production of high-titer lentiviral particles and easy establishment of stable cell lines. All of our ORF cDNA constructs feature an epitope tag (e.g. Flag, V5, Myc, HA, etc.) fused to the ORF C-terminus, allowing easy purification or staining of fixed cells/lysates. In live cells, drug selection markers allow stable cell line generation in ~10 days; or, fluorescent reporter can allow isolation by FACS.  Track cells in vivo or in vitro using fluorescence or luciferase. Self-cleaving peptides are included to allow independent protein expression of these components from a single mRNA transcript.
• High-titer & ultra purified – LipExoGen  lentiviral particles are ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation. They are suitable for transduction of primary cells, stem cells, and difficult-to-transfect cells including primary or thawed cells.
• 100% Customizable – LipExoGen lentiviral particles can be customized according to your requirements for a price that is comparable to that shown on the website.

For immunotherapy/functional assay reporter lentiviruses, if the receptor cDNA ORF exceeds our 1.2 kb packaging capacity for an all-in-one construct, transduction efficiency will be significantly reduced. In such cases, we provide the cDNA ORF lentivirus and the reporter lentivirus as a set instead. You can use this set for co-transfection, ensuring high-efficiency transduction without additional time delays. The price for this set is $1200.